Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28449 | 5' | -59.4 | NC_005946.1 | + | 41423 | 0.66 | 0.68309 |
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Target: 5'- --aGGUCCGcGGGGUCCCGGcGGa-- -3' miRNA: 3'- uucCCAGGUuCCCCAGGGUUcCCacc -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 102889 | 0.67 | 0.611241 |
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Target: 5'- cAGGGGUCCAGaGGGaCCCAgaggaccggAGGGagcuccUGGa -3' miRNA: 3'- -UUCCCAGGUUcCCCaGGGU---------UCCC------ACC- -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 88171 | 0.67 | 0.579506 |
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Target: 5'- gGAGGGUaguGGGGGUaccguggcucaggCCCuguccAGGGUGGu -3' miRNA: 3'- -UUCCCAgguUCCCCA-------------GGGu----UCCCACC- -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 19710 | 0.69 | 0.51045 |
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Target: 5'- uGAGGGU-CAAGGGGgagaggCUCAAGGaGUGc -3' miRNA: 3'- -UUCCCAgGUUCCCCa-----GGGUUCC-CACc -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 21856 | 0.69 | 0.491052 |
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Target: 5'- cAAGGaGUCCAuGGGG---CAGGGGUGGc -3' miRNA: 3'- -UUCC-CAGGUuCCCCaggGUUCCCACC- -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 76374 | 0.71 | 0.374693 |
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Target: 5'- -uGGGUCCAAccGGUCCCAgcGGGGcgccUGGa -3' miRNA: 3'- uuCCCAGGUUccCCAGGGU--UCCC----ACC- -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 13325 | 0.71 | 0.366542 |
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Target: 5'- uGGGGUCCAGGGuGGUggCCGAGGGc-- -3' miRNA: 3'- uUCCCAGGUUCC-CCAg-GGUUCCCacc -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 76650 | 0.72 | 0.35061 |
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Target: 5'- cAGGGGcCCA-GGGG-CCCAGGGGa-- -3' miRNA: 3'- -UUCCCaGGUuCCCCaGGGUUCCCacc -5' |
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| 28449 | 5' | -59.4 | NC_005946.1 | + | 103080 | 1.08 | 0.000998 |
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Target: 5'- gAAGGGUCCAAGGGGUCCCAAGGGUGGa -3' miRNA: 3'- -UUCCCAGGUUCCCCAGGGUUCCCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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