Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28450 | 3' | -52.4 | NC_005946.1 | + | 42395 | 0.66 | 0.961126 |
Target: 5'- gGGUcuUUGgGACcuCUGUCUCCCGGGAc- -3' miRNA: 3'- -UCA--AAUgUUGc-GGCAGAGGGUCCUcc -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 80411 | 0.66 | 0.957345 |
Target: 5'- gAGUcguCGACccucuuGCCGUCUCCCGcaaaGAGGa -3' miRNA: 3'- -UCAaauGUUG------CGGCAGAGGGUc---CUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 12545 | 0.66 | 0.949047 |
Target: 5'- ----gGCaAGCGCCaugC-CCCAGGAGGu -3' miRNA: 3'- ucaaaUG-UUGCGGca-GaGGGUCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 72851 | 0.68 | 0.911957 |
Target: 5'- ----aACAugGCCGagggggcgggguUCUCCCuGGAGa -3' miRNA: 3'- ucaaaUGUugCGGC------------AGAGGGuCCUCc -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 3032 | 0.69 | 0.885111 |
Target: 5'- -----cCGACGUCGUCccggccUCCAGGAGGg -3' miRNA: 3'- ucaaauGUUGCGGCAGa-----GGGUCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 50009 | 0.69 | 0.877781 |
Target: 5'- aGGgcUGCGAaaGCCGcagaCUCCCAGGuGGg -3' miRNA: 3'- -UCaaAUGUUg-CGGCa---GAGGGUCCuCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 23804 | 0.69 | 0.867897 |
Target: 5'- -----cCAGCGUgGUCUggagggacugcaggCCCAGGAGGa -3' miRNA: 3'- ucaaauGUUGCGgCAGA--------------GGGUCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 22126 | 0.7 | 0.846142 |
Target: 5'- --cUUGCAGCGCCGUCagaacacucUCUCucGAGGg -3' miRNA: 3'- ucaAAUGUUGCGGCAG---------AGGGucCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 73414 | 0.7 | 0.837686 |
Target: 5'- ----cGCAACGCCaagggCUCCaAGGAGGa -3' miRNA: 3'- ucaaaUGUUGCGGca---GAGGgUCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 56356 | 0.7 | 0.829027 |
Target: 5'- cGUggUACAugGCCGcCagCCuGGAGGa -3' miRNA: 3'- uCAa-AUGUugCGGCaGagGGuCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 36622 | 0.7 | 0.811135 |
Target: 5'- ---cUGgGACGCCGUCUCCCAugccGAGu -3' miRNA: 3'- ucaaAUgUUGCGGCAGAGGGUc---CUCc -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 71551 | 0.7 | 0.801919 |
Target: 5'- ---aUGCGGaGCUGUCcugaCCCAGGAGGa -3' miRNA: 3'- ucaaAUGUUgCGGCAGa---GGGUCCUCC- -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 2641 | 0.71 | 0.763487 |
Target: 5'- gGGgcUGCAGCGCCuUCacaCCCAGGGGc -3' miRNA: 3'- -UCaaAUGUUGCGGcAGa--GGGUCCUCc -5' |
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28450 | 3' | -52.4 | NC_005946.1 | + | 104485 | 1.12 | 0.002989 |
Target: 5'- cAGUUUACAACGCCGUCUCCCAGGAGGg -3' miRNA: 3'- -UCAAAUGUUGCGGCAGAGGGUCCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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