Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28450 | 5' | -56.5 | NC_005946.1 | + | 66287 | 0.66 | 0.825165 |
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Target: 5'- uGACCUCCUCg-----GAC-CCUUCCCu -3' miRNA: 3'- -UUGGAGGAGguccaaCUGcGGAAGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 924 | 0.66 | 0.816364 |
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Target: 5'- -uCCUUUUCCAGGUgcUGAaUGUCUaCCCu -3' miRNA: 3'- uuGGAGGAGGUCCA--ACU-GCGGAaGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 61863 | 0.66 | 0.798241 |
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Target: 5'- -cCCUCCUCCc----GACGCCcugCCCa -3' miRNA: 3'- uuGGAGGAGGuccaaCUGCGGaa-GGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 69792 | 0.66 | 0.788938 |
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Target: 5'- uGGCCUUUUCCAGGUcccUGAUcuuguaGUCU-CCCa -3' miRNA: 3'- -UUGGAGGAGGUCCA---ACUG------CGGAaGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 63592 | 0.66 | 0.779485 |
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Target: 5'- -cUCUCCUCCAGGgaa--GgUUUCCCa -3' miRNA: 3'- uuGGAGGAGGUCCaacugCgGAAGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 8550 | 0.67 | 0.750332 |
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Target: 5'- cACCUcguccaCCUCCAGGgUGcCGUCUggaCCCa -3' miRNA: 3'- uUGGA------GGAGGUCCaACuGCGGAa--GGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 28922 | 0.67 | 0.720193 |
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Target: 5'- ----gCCUCCAGGcacUUGACGaCCUgCCCu -3' miRNA: 3'- uuggaGGAGGUCC---AACUGC-GGAaGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 90980 | 0.69 | 0.626523 |
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Target: 5'- cAACCUCCUCguGGUU--C-CCUUCCUa -3' miRNA: 3'- -UUGGAGGAGguCCAAcuGcGGAAGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 34382 | 0.69 | 0.605527 |
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Target: 5'- gGGCCcCCUCUcgcccacgguucGGGgaGACGCCUgCCCu -3' miRNA: 3'- -UUGGaGGAGG------------UCCaaCUGCGGAaGGG- -5' |
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| 28450 | 5' | -56.5 | NC_005946.1 | + | 104451 | 1.1 | 0.001302 |
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Target: 5'- gAACCUCCUCCAGGUUGACGCCUUCCCa -3' miRNA: 3'- -UUGGAGGAGGUCCAACUGCGGAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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