Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28452 | 5' | -56.1 | NC_005946.1 | + | 2829 | 0.7 | 0.566416 |
Target: 5'- gUCCAAggGGUCCCaaggGUGGAGUGGGAg-- -3' miRNA: 3'- -GGGUU--UCGGGGg---CACCUCACCUUguu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 6944 | 0.69 | 0.629314 |
Target: 5'- aCCAGAGCCCUgauUGUGGGGUGcaGGugAGa -3' miRNA: 3'- gGGUUUCGGGG---GCACCUCAC--CUugUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 13616 | 0.67 | 0.782416 |
Target: 5'- cUCCAGA-CCCCUGaGGAGUGuGGACu- -3' miRNA: 3'- -GGGUUUcGGGGGCaCCUCAC-CUUGuu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 22949 | 0.67 | 0.782416 |
Target: 5'- cCCCucAGCCCCgGcGGGGaUGGAAa-- -3' miRNA: 3'- -GGGuuUCGGGGgCaCCUC-ACCUUguu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 23039 | 0.68 | 0.681853 |
Target: 5'- gUCCAGGGUCCCCGUGGGaccuGUccacGGGucGCAGa -3' miRNA: 3'- -GGGUUUCGGGGGCACCU----CA----CCU--UGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 25973 | 0.68 | 0.723155 |
Target: 5'- gCCGAGGCgCCCaCGgcGGGG-GGGACAAu -3' miRNA: 3'- gGGUUUCG-GGG-GCa-CCUCaCCUUGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 33391 | 0.73 | 0.401588 |
Target: 5'- uCCCccGGCCCCCGaGGAGaGGGAUAu -3' miRNA: 3'- -GGGuuUCGGGGGCaCCUCaCCUUGUu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 43495 | 0.66 | 0.819198 |
Target: 5'- uUCCAuGGaCCCCGcGGGGUucgaGGAGCAGg -3' miRNA: 3'- -GGGUuUCgGGGGCaCCUCA----CCUUGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 45763 | 0.69 | 0.671394 |
Target: 5'- gUCAGGGCUgCCGUGGuGGUGGggUc- -3' miRNA: 3'- gGGUUUCGGgGGCACC-UCACCuuGuu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 56470 | 0.66 | 0.81025 |
Target: 5'- cUCCAGGGCCC---UGGAG-GGGACGGg -3' miRNA: 3'- -GGGUUUCGGGggcACCUCaCCUUGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 58838 | 0.71 | 0.525356 |
Target: 5'- cCCCAucauGCCCCUGUGGcggugAGUGGccACAAa -3' miRNA: 3'- -GGGUuu--CGGGGGCACC-----UCACCu-UGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 61675 | 0.67 | 0.781464 |
Target: 5'- uCCCAGAGCUCCC-UGGcgcucacAGUGuGAGCc- -3' miRNA: 3'- -GGGUUUCGGGGGcACC-------UCAC-CUUGuu -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 86091 | 0.67 | 0.782416 |
Target: 5'- cCCCAggacaggcucAGGCCCaCCGUGGAGa--AGCAGg -3' miRNA: 3'- -GGGU----------UUCGGG-GGCACCUCaccUUGUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 95262 | 0.67 | 0.753294 |
Target: 5'- cCCCAccAGGUcccucaccucauCCCCG-GGAGUGGAGgGGu -3' miRNA: 3'- -GGGU--UUCG------------GGGGCaCCUCACCUUgUU- -5' |
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28452 | 5' | -56.1 | NC_005946.1 | + | 97730 | 0.69 | 0.629314 |
Target: 5'- aCC--AGCCUCC-UGGGGUGGAACc- -3' miRNA: 3'- gGGuuUCGGGGGcACCUCACCUUGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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