Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28453 | 5' | -59.4 | NC_005946.1 | + | 6575 | 0.68 | 0.519197 |
Target: 5'- -cGCUCCUCCGGGUCaaagaccaUaaaaacauaucccaGGUCCUUGUc -3' miRNA: 3'- gaCGAGGGGGUCCAGg-------G--------------UCAGGAACG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 11796 | 0.68 | 0.542017 |
Target: 5'- uCUG-UCCUCCAGGaUCCCAGcUCCcgccgGCc -3' miRNA: 3'- -GACgAGGGGGUCC-AGGGUC-AGGaa---CG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 33151 | 0.67 | 0.58243 |
Target: 5'- -cGCUCCCgCCAGGcuggCgUGGUCCggcgcUGCg -3' miRNA: 3'- gaCGAGGG-GGUCCa---GgGUCAGGa----ACG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 33430 | 0.66 | 0.654249 |
Target: 5'- -cGCUCCCuCCucGUCCCcGUcggacucucucCCUUGCc -3' miRNA: 3'- gaCGAGGG-GGucCAGGGuCA-----------GGAACG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 37070 | 0.7 | 0.436855 |
Target: 5'- -aGCUCCCuggccucagaguCCAGGUCCCAcagcUCCgaGCu -3' miRNA: 3'- gaCGAGGG------------GGUCCAGGGUc---AGGaaCG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 56966 | 0.69 | 0.512327 |
Target: 5'- -cGUUCCUCaCGGGUCCCcuGGUCCa--- -3' miRNA: 3'- gaCGAGGGG-GUCCAGGG--UCAGGaacg -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 57204 | 0.66 | 0.643977 |
Target: 5'- --cCUCCUCCAccuGUCCCAGUCCa--- -3' miRNA: 3'- gacGAGGGGGUc--CAGGGUCAGGaacg -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 64220 | 0.66 | 0.684921 |
Target: 5'- -gGCUCCCCCuGaGUCCCAcGgggCCa--- -3' miRNA: 3'- gaCGAGGGGGuC-CAGGGU-Ca--GGaacg -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 70574 | 0.69 | 0.473838 |
Target: 5'- -aGCgUCUCCCAGGUCCCA-UCCcagggagGCc -3' miRNA: 3'- gaCG-AGGGGGUCCAGGGUcAGGaa-----CG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 74063 | 0.66 | 0.684921 |
Target: 5'- -aGCUUCUCCaucAGGUCCCu-UCCcUGCu -3' miRNA: 3'- gaCGAGGGGG---UCCAGGGucAGGaACG- -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 83826 | 0.67 | 0.633697 |
Target: 5'- --cCUCCCCCuugauGGUCCUgccGGUCCUg-- -3' miRNA: 3'- gacGAGGGGGu----CCAGGG---UCAGGAacg -5' |
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28453 | 5' | -59.4 | NC_005946.1 | + | 92963 | 0.66 | 0.683904 |
Target: 5'- gUGCUCCaacguguCCguGGagCCCGGUCCccUGCc -3' miRNA: 3'- gACGAGG-------GGguCCa-GGGUCAGGa-ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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