Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28454 | 3' | -62.8 | NC_005946.1 | + | 38361 | 0.66 | 0.493918 |
Target: 5'- ---cUGGGCCcugcacUGGUGGCaggcgaAGCCGGCc -3' miRNA: 3'- cgcaGCCCGG------AUCACCGgg----UCGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 71250 | 0.66 | 0.50049 |
Target: 5'- -gGUCGGGCCUggacauggauaucuGGagGGCCCucAGCCucuGGUu -3' miRNA: 3'- cgCAGCCCGGA--------------UCa-CCGGG--UCGG---CCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 66064 | 0.66 | 0.512796 |
Target: 5'- cUGUCuaGGGCuCUGGcuaUGGCCgCGGCugCGGCg -3' miRNA: 3'- cGCAG--CCCG-GAUC---ACCGG-GUCG--GCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 97684 | 0.66 | 0.531964 |
Target: 5'- gGCGUUGGGUCUgcagcAGggaGGCa-GGaCCGGCa -3' miRNA: 3'- -CGCAGCCCGGA-----UCa--CCGggUC-GGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 102447 | 0.66 | 0.531964 |
Target: 5'- cCGgggCGGGUgUAGgGGUCUAcGCCGGUc -3' miRNA: 3'- cGCa--GCCCGgAUCaCCGGGU-CGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 69873 | 0.66 | 0.534863 |
Target: 5'- cGCGUCagugguuccucccuGGCCUcauacggAGUGGCCUccagGGCCucGGCg -3' miRNA: 3'- -CGCAGc-------------CCGGA-------UCACCGGG----UCGG--CCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 41178 | 0.66 | 0.541646 |
Target: 5'- aGCG-CGGGCCcgucAGcccaaccuGUCCGGCCGGUc -3' miRNA: 3'- -CGCaGCCCGGa---UCac------CGGGUCGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 8040 | 0.67 | 0.448206 |
Target: 5'- cCGUCcuGGCCU-GUGGCCgGGgCGGg -3' miRNA: 3'- cGCAGc-CCGGAuCACCGGgUCgGCCg -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 29502 | 0.67 | 0.466219 |
Target: 5'- aUGagGGGCCUAGaGGCCC--CCGGa -3' miRNA: 3'- cGCagCCCGGAUCaCCGGGucGGCCg -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 29333 | 0.68 | 0.3966 |
Target: 5'- -gGUCGGGCCcgUGGgagcGGCUgGGCCucaggGGCa -3' miRNA: 3'- cgCAGCCCGG--AUCa---CCGGgUCGG-----CCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 69430 | 0.68 | 0.421928 |
Target: 5'- cCGg-GGGuCCUGGUcccaaacggcagGGCCCaAGCCGGUc -3' miRNA: 3'- cGCagCCC-GGAUCA------------CCGGG-UCGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 84584 | 0.69 | 0.364391 |
Target: 5'- uGUGUCcaGGGCU---UGGCCgaggaGGCCGGCa -3' miRNA: 3'- -CGCAG--CCCGGaucACCGGg----UCGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 46312 | 0.7 | 0.31957 |
Target: 5'- -aGagGGGuCCUA-UGGCCCuccuGCCGGCc -3' miRNA: 3'- cgCagCCC-GGAUcACCGGGu---CGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 79239 | 0.71 | 0.272667 |
Target: 5'- uCGUCGGGCUUGcUGGCCCcguacaccAGCCaGGg -3' miRNA: 3'- cGCAGCCCGGAUcACCGGG--------UCGG-CCg -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 26393 | 0.71 | 0.285478 |
Target: 5'- gGCGUUguagugccuggaGGGCCUGacGGCCgAGCUGGUg -3' miRNA: 3'- -CGCAG------------CCCGGAUcaCCGGgUCGGCCG- -5' |
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28454 | 3' | -62.8 | NC_005946.1 | + | 59937 | 0.75 | 0.145411 |
Target: 5'- gGCGUCGGGCCgauugccGGUGagaGCaguaagCCAGCUGGCg -3' miRNA: 3'- -CGCAGCCCGGa------UCAC---CG------GGUCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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