Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28458 | 5' | -56.1 | NC_005946.1 | + | 98080 | 0.67 | 0.764699 |
Target: 5'- aAGUGuCCCGGG-CACUcacguccgguguuuUcgcacguUGGGCACAg- -3' miRNA: 3'- -UCAC-GGGCCCuGUGA--------------A-------ACCCGUGUau -5' |
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28458 | 5' | -56.1 | NC_005946.1 | + | 48109 | 0.67 | 0.758765 |
Target: 5'- -cUGUuuGaGGACagggGCUUUGGGUACGUGu -3' miRNA: 3'- ucACGggC-CCUG----UGAAACCCGUGUAU- -5' |
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28458 | 5' | -56.1 | NC_005946.1 | + | 25353 | 0.67 | 0.758765 |
Target: 5'- ---uCCCGGGGCACUUgUGGcaGCACAc- -3' miRNA: 3'- ucacGGGCCCUGUGAA-ACC--CGUGUau -5' |
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28458 | 5' | -56.1 | NC_005946.1 | + | 92972 | 0.68 | 0.711989 |
Target: 5'- cGUGUCCGuGGAgcccgguccccugccCGCUuugUUGGGCGCGUc -3' miRNA: 3'- uCACGGGC-CCU---------------GUGA---AACCCGUGUAu -5' |
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28458 | 5' | -56.1 | NC_005946.1 | + | 44702 | 0.68 | 0.655163 |
Target: 5'- cAGUGCCUGGGcAgGCUgaGGGC-CAa- -3' miRNA: 3'- -UCACGGGCCC-UgUGAaaCCCGuGUau -5' |
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28458 | 5' | -56.1 | NC_005946.1 | + | 4093 | 0.72 | 0.468696 |
Target: 5'- uAGUGCCaCGGGACGCUgu-GGCAg--- -3' miRNA: 3'- -UCACGG-GCCCUGUGAaacCCGUguau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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