Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28460 | 5' | -55.4 | NC_005946.1 | + | 102048 | 0.68 | 0.730876 |
Target: 5'- uGUCGuGGAGGaUCCCCGGCGucuGCAGg -3' miRNA: 3'- -CAGU-UCUCCgAGGGGCCGUuc-UGUUa -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 92210 | 0.66 | 0.836136 |
Target: 5'- -cUAGGcAGuGCUCCCUGGCGgucaugccAGACAGg -3' miRNA: 3'- caGUUC-UC-CGAGGGGCCGU--------UCUGUUa -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 84734 | 0.77 | 0.263342 |
Target: 5'- uUCAAGAGGCUCCUgGGaAAGACGGc -3' miRNA: 3'- cAGUUCUCCGAGGGgCCgUUCUGUUa -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 80297 | 0.7 | 0.624956 |
Target: 5'- -cCAGGAGGCugUCCCUGGaCAAGcACAu- -3' miRNA: 3'- caGUUCUCCG--AGGGGCC-GUUC-UGUua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 65766 | 0.77 | 0.238143 |
Target: 5'- --gGAGAGGCUCUCCGGCGGGcAUAAa -3' miRNA: 3'- cagUUCUCCGAGGGGCCGUUC-UGUUa -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 63931 | 0.67 | 0.771198 |
Target: 5'- cUCAAGAG--UCCCCGGguGGACc-- -3' miRNA: 3'- cAGUUCUCcgAGGGGCCguUCUGuua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 61630 | 0.68 | 0.730876 |
Target: 5'- uUCAGGuaGGGCUCCCCGGUccuGAa--- -3' miRNA: 3'- cAGUUC--UCCGAGGGGCCGuu-CUguua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 34619 | 0.67 | 0.790584 |
Target: 5'- -gCAGGAGGCUCCCCacaAAGAUc-- -3' miRNA: 3'- caGUUCUCCGAGGGGccgUUCUGuua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 26570 | 0.66 | 0.844672 |
Target: 5'- gGUCGAGGGGUcagagucggCCCUGGauaAAGACGc- -3' miRNA: 3'- -CAGUUCUCCGa--------GGGGCCg--UUCUGUua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 26444 | 0.67 | 0.771198 |
Target: 5'- cGUCGAGGGGUgggCCGGguGGACGc- -3' miRNA: 3'- -CAGUUCUCCGaggGGCCguUCUGUua -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 25975 | 0.66 | 0.827397 |
Target: 5'- ----cGAGGCgCCCaCGGCGggggGGACAAUg -3' miRNA: 3'- caguuCUCCGaGGG-GCCGU----UCUGUUA- -5' |
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28460 | 5' | -55.4 | NC_005946.1 | + | 10575 | 0.66 | 0.809343 |
Target: 5'- ----cGAGGCgauuucCCCCGGCGcccguGGACGAg -3' miRNA: 3'- caguuCUCCGa-----GGGGCCGU-----UCUGUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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