Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28461 | 3' | -56 | NC_005946.1 | + | 87134 | 0.66 | 0.859751 |
Target: 5'- aCCCUCUUCgUCAucGCuaGgGCCaucUUCGUCa -3' miRNA: 3'- -GGGAGAAGgAGUu-CG--UgCGG---AGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 40845 | 0.66 | 0.843668 |
Target: 5'- gUCCUCggaCUCGAGCAgGU--CCGUCa -3' miRNA: 3'- -GGGAGaagGAGUUCGUgCGgaGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 32340 | 0.66 | 0.826783 |
Target: 5'- gCCC---UCCUCAuggagcAGCACGCgUCCGcCc -3' miRNA: 3'- -GGGagaAGGAGU------UCGUGCGgAGGCaG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 16725 | 0.66 | 0.826783 |
Target: 5'- gCCUCUgcucgcacaUCCUCAGGCuCGCgaaacgUCCGUg -3' miRNA: 3'- gGGAGA---------AGGAGUUCGuGCGg-----AGGCAg -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 59887 | 0.66 | 0.826783 |
Target: 5'- aCCUUgUCCUCGGGCGCcuccccgacaGCCU-UGUCa -3' miRNA: 3'- gGGAGaAGGAGUUCGUG----------CGGAgGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 71279 | 0.67 | 0.80916 |
Target: 5'- gCCCUCagCCUCu-GguUGuCCUCCGUg -3' miRNA: 3'- -GGGAGaaGGAGuuCguGC-GGAGGCAg -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 62756 | 0.67 | 0.80826 |
Target: 5'- gCCUUgacgaCCUCGAGCGCauaagguuuggaaGCCgCCGUCc -3' miRNA: 3'- gGGAGaa---GGAGUUCGUG-------------CGGaGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 9634 | 0.67 | 0.800092 |
Target: 5'- aCCCUCUcgggcUCCUCAAagACGUCgUCGUCc -3' miRNA: 3'- -GGGAGA-----AGGAGUUcgUGCGGaGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 63968 | 0.67 | 0.800092 |
Target: 5'- gCCCUUUggcCCUCuggGGCcaucccuaccaAUGUCUCCGUCu -3' miRNA: 3'- -GGGAGAa--GGAGu--UCG-----------UGCGGAGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 31407 | 0.67 | 0.790866 |
Target: 5'- cUCCUCggCCUCGAcCugGUugacugaaucgaCUCCGUCg -3' miRNA: 3'- -GGGAGaaGGAGUUcGugCG------------GAGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 96280 | 0.67 | 0.790866 |
Target: 5'- cCCCg--UCCUCGucauccGC-CGCCUCCGcCu -3' miRNA: 3'- -GGGagaAGGAGUu-----CGuGCGGAGGCaG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 96348 | 0.69 | 0.681651 |
Target: 5'- aCCgUC-UCCUCGGGC-C-UCUCCGUCa -3' miRNA: 3'- -GGgAGaAGGAGUUCGuGcGGAGGCAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 78104 | 0.7 | 0.619065 |
Target: 5'- gCCUCccgucCCUCAGGCACcCCUCCcuaaaGUCg -3' miRNA: 3'- gGGAGaa---GGAGUUCGUGcGGAGG-----CAG- -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 31973 | 0.71 | 0.567103 |
Target: 5'- aCCUCUUCCgggaGAGUGCcCCUCCGa- -3' miRNA: 3'- gGGAGAAGGag--UUCGUGcGGAGGCag -5' |
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28461 | 3' | -56 | NC_005946.1 | + | 37055 | 0.72 | 0.516318 |
Target: 5'- uCCCUCUcccUCCUCAGcuccCugGCCUCagaGUCc -3' miRNA: 3'- -GGGAGA---AGGAGUUc---GugCGGAGg--CAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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