Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28461 | 5' | -58.4 | NC_005946.1 | + | 81197 | 0.66 | 0.785495 |
Target: 5'- cGGuuUGGGGGggUuggGCCCGgGUGGGGc -3' miRNA: 3'- cCCucGCCUCCuuG---UGGGCaCGCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 101619 | 0.66 | 0.785495 |
Target: 5'- uGGGuGUGGAaGGACACgaggCCGU-CGGGGg -3' miRNA: 3'- -CCCuCGCCUcCUUGUG----GGCAcGCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 69492 | 0.66 | 0.77632 |
Target: 5'- aGGGGCacGGAGGGACGCggaaCC-UGCGGGc -3' miRNA: 3'- cCCUCG--CCUCCUUGUG----GGcACGCUCc -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 103307 | 0.66 | 0.77632 |
Target: 5'- uGGGAcCuGGGGGAGCAgaCGUGCGccucGGGc -3' miRNA: 3'- -CCCUcG-CCUCCUUGUggGCACGC----UCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 44584 | 0.67 | 0.709037 |
Target: 5'- -uGAGUGGgacAGGGACGCCUgGUGUGuGGu -3' miRNA: 3'- ccCUCGCC---UCCUUGUGGG-CACGCuCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 71487 | 0.67 | 0.699096 |
Target: 5'- uGGGAcCGGAGcacauGGACACCaCGggaacGUGAGGg -3' miRNA: 3'- -CCCUcGCCUC-----CUUGUGG-GCa----CGCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 33983 | 0.67 | 0.689101 |
Target: 5'- uGGGAGacgaCGGGGuGAuAgACCCGUcgugcGCGGGGg -3' miRNA: 3'- -CCCUC----GCCUC-CU-UgUGGGCA-----CGCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 93307 | 0.67 | 0.683081 |
Target: 5'- aGGAgGUGGAGGAGCuccugacggugucuCCCGUG-GAGa -3' miRNA: 3'- cCCU-CGCCUCCUUGu-------------GGGCACgCUCc -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 10396 | 0.68 | 0.668979 |
Target: 5'- aGGAgGCGGAGGAcgACCCGccgcagGUGGGa -3' miRNA: 3'- cCCU-CGCCUCCUugUGGGCa-----CGCUCc -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 53600 | 0.68 | 0.638596 |
Target: 5'- aGGGuaUGGAGGGAgACCCgGUGCaGAGa -3' miRNA: 3'- -CCCucGCCUCCUUgUGGG-CACG-CUCc -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 29522 | 0.68 | 0.628448 |
Target: 5'- cGGAGCGGuGGGuccaACCgGUcccaGCGGGGc -3' miRNA: 3'- cCCUCGCCuCCUug--UGGgCA----CGCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 33063 | 0.69 | 0.59805 |
Target: 5'- aGGGGGCGGGguucucccuGGAGaCACCCa-GgGAGGu -3' miRNA: 3'- -CCCUCGCCU---------CCUU-GUGGGcaCgCUCC- -5' |
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28461 | 5' | -58.4 | NC_005946.1 | + | 41940 | 0.69 | 0.567872 |
Target: 5'- aGGGAcGCGGGGGGuaucucugACCCGUcuGCGAuGGc -3' miRNA: 3'- -CCCU-CGCCUCCUug------UGGGCA--CGCU-CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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