Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28465 | 3' | -58.5 | NC_005946.1 | + | 78077 | 0.66 | 0.743586 |
Target: 5'- -gGGCCCUCCUCGacagcuuguccucgGGCcuccCGUCCc- -3' miRNA: 3'- ugCCGGGAGGAGUa-------------CCGuu--GCAGGcu -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 88521 | 0.66 | 0.719865 |
Target: 5'- uACGGCCCUUUaCA-GGaCAAUGUuuGAg -3' miRNA: 3'- -UGCCGGGAGGaGUaCC-GUUGCAggCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 41972 | 0.66 | 0.699755 |
Target: 5'- gAUGGCCCUcagCCUCu--GCAcuGCGUCCa- -3' miRNA: 3'- -UGCCGGGA---GGAGuacCGU--UGCAGGcu -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 21823 | 0.67 | 0.68961 |
Target: 5'- -aGGCCUacucggUCCUCAUGGCGuCuUCUGGa -3' miRNA: 3'- ugCCGGG------AGGAGUACCGUuGcAGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 8427 | 0.67 | 0.658918 |
Target: 5'- -gGGUagugCUCCUugacaaaguaCGUGGUGGCGUCCGAa -3' miRNA: 3'- ugCCGg---GAGGA----------GUACCGUUGCAGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 86125 | 0.68 | 0.601274 |
Target: 5'- -aGGCCgUCCuccugucccuggugaUCAUGGCAGCccCCGAc -3' miRNA: 3'- ugCCGGgAGG---------------AGUACCGUUGcaGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 33148 | 0.68 | 0.586923 |
Target: 5'- -gGGCgCUCCcgcCAggcUGGCGugGUCCGGc -3' miRNA: 3'- ugCCGgGAGGa--GU---ACCGUugCAGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 6179 | 0.69 | 0.516502 |
Target: 5'- cAC-GCCCUCUcCAUGGCcacucuGGCGUCUGAa -3' miRNA: 3'- -UGcCGGGAGGaGUACCG------UUGCAGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 3741 | 0.72 | 0.387969 |
Target: 5'- -gGGaCCUCCUCAUGaGCucguaguCGUCCGAg -3' miRNA: 3'- ugCCgGGAGGAGUAC-CGuu-----GCAGGCU- -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 78962 | 0.72 | 0.355276 |
Target: 5'- gGCGGgCUUCCUCugccUGGCcACGUCCa- -3' miRNA: 3'- -UGCCgGGAGGAGu---ACCGuUGCAGGcu -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 67224 | 0.73 | 0.33206 |
Target: 5'- uCGGCCUUCCUCAUGGUAGaguaUCCu- -3' miRNA: 3'- uGCCGGGAGGAGUACCGUUgc--AGGcu -5' |
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28465 | 3' | -58.5 | NC_005946.1 | + | 32336 | 0.87 | 0.038699 |
Target: 5'- gGCGGCCCUCCUCAUGGagcagcacGCGUCCGc -3' miRNA: 3'- -UGCCGGGAGGAGUACCgu------UGCAGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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