Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28467 | 3' | -53 | NC_005946.1 | + | 42086 | 0.66 | 0.947932 |
Target: 5'- aCCacagGC-CCUCUUGAcGUAGggucuggggacCAGGAGCu -3' miRNA: 3'- -GGa---CGcGGAGAACUaCGUU-----------GUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 70003 | 0.66 | 0.93844 |
Target: 5'- uCCUG-GCCgucCUgagGAUGCAAgacacCAGGcAGCa -3' miRNA: 3'- -GGACgCGGa--GAa--CUACGUU-----GUCC-UCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 96781 | 0.66 | 0.932775 |
Target: 5'- cCCUG-GCgCUCaUGAUGacgcucuuugguuCAugAGGAGCa -3' miRNA: 3'- -GGACgCG-GAGaACUAC-------------GUugUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 50972 | 0.67 | 0.916318 |
Target: 5'- gCCUGaggagGCUgag-GAUGCugAGCAGGAGCc -3' miRNA: 3'- -GGACg----CGGagaaCUACG--UUGUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 23544 | 0.67 | 0.896301 |
Target: 5'- aCCacgGCGuCCUCggccgagagGAUGgacguguCAGCGGGAGCg -3' miRNA: 3'- -GGa--CGC-GGAGaa-------CUAC-------GUUGUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 11143 | 0.67 | 0.89003 |
Target: 5'- aCCaGCGCCcCUUGG-GUGGCGGGucacAGCa -3' miRNA: 3'- -GGaCGCGGaGAACUaCGUUGUCC----UCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 44890 | 0.68 | 0.851618 |
Target: 5'- gCCUGCGCCUCgaacccGAcGCcACAGaGGUa -3' miRNA: 3'- -GGACGCGGAGaa----CUaCGuUGUCcUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 80950 | 0.69 | 0.825896 |
Target: 5'- --gGcCGCCUCcc-GUGCuAGCGGGAGCg -3' miRNA: 3'- ggaC-GCGGAGaacUACG-UUGUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 41516 | 0.7 | 0.798434 |
Target: 5'- gCCUGCaCCUCUU--UGCGAUGGG-GCc -3' miRNA: 3'- -GGACGcGGAGAAcuACGUUGUCCuCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 66540 | 0.7 | 0.749497 |
Target: 5'- cCCUGCGUCUUcUGAUcUGGCGGGGGa -3' miRNA: 3'- -GGACGCGGAGaACUAcGUUGUCCUCg -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 37695 | 0.72 | 0.644441 |
Target: 5'- aCUUGUagucCCUCUUGAUG-AugGGGAGCa -3' miRNA: 3'- -GGACGc---GGAGAACUACgUugUCCUCG- -5' |
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28467 | 3' | -53 | NC_005946.1 | + | 9377 | 0.73 | 0.621911 |
Target: 5'- uCUUGCuCaUCUUGAUGCGagagccuGCAGGAGCc -3' miRNA: 3'- -GGACGcGgAGAACUACGU-------UGUCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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