Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28468 | 3' | -50.3 | NC_005946.1 | + | 36941 | 0.66 | 0.991685 |
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Target: 5'- aGCUgguUCUUGAgGGCGG-CUCUgguggUCCu -3' miRNA: 3'- -CGA---AGAGCUgCCGUCuGAGAaag--AGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 16220 | 0.67 | 0.981967 |
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Target: 5'- gGCccUCUUGAUGGaaaAGACauUCUUgggCUCCa -3' miRNA: 3'- -CGa-AGAGCUGCCg--UCUG--AGAAa--GAGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 97569 | 0.67 | 0.974695 |
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Target: 5'- ---cUUCGACGGCGGGguuaUCgggCUCCa -3' miRNA: 3'- cgaaGAGCUGCCGUCUg---AGaaaGAGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 3886 | 0.68 | 0.968787 |
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Target: 5'- gGCUgccagCUCGGgGGCcuGCUCgc-CUCCg -3' miRNA: 3'- -CGAa----GAGCUgCCGucUGAGaaaGAGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 66015 | 0.68 | 0.968787 |
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Target: 5'- gGCUgaUCUgCGACGcacuGCAGGCUCgucgUUC-CCg -3' miRNA: 3'- -CGA--AGA-GCUGC----CGUCUGAGa---AAGaGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 70422 | 0.68 | 0.961958 |
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Target: 5'- aGCUUCUCGgacaggaucACGGCcgcuguGGACUCUgggUCagacgcugccacUCCa -3' miRNA: 3'- -CGAAGAGC---------UGCCG------UCUGAGAa--AG------------AGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 30206 | 0.71 | 0.883235 |
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Target: 5'- aGgaUgUCGGCGGCcucucccuuguugaAGACUCUUUCUgCu -3' miRNA: 3'- -CgaAgAGCUGCCG--------------UCUGAGAAAGAgG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 71126 | 0.73 | 0.800167 |
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Target: 5'- gGCUUggCGGCGGCGGGgUagggggaCUUUCUCCc -3' miRNA: 3'- -CGAAgaGCUGCCGUCUgA-------GAAAGAGG- -5' |
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| 28468 | 3' | -50.3 | NC_005946.1 | + | 11365 | 0.83 | 0.318018 |
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Target: 5'- aGCUUUggagCGACGGCGGugUCaucgUCUCCu -3' miRNA: 3'- -CGAAGa---GCUGCCGUCugAGaa--AGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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