Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28471 | 3' | -58.1 | NC_005946.1 | + | 78090 | 0.66 | 0.735057 |
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Target: 5'- cAGCuugucCUCggGCCUCCCGUCccucaGGCAc-- -3' miRNA: 3'- -UCGu----GAGaaCGGAGGGCAG-----CCGUcuc -5' |
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| 28471 | 3' | -58.1 | NC_005946.1 | + | 79738 | 0.67 | 0.674017 |
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Target: 5'- --gGCUCUggugagugaGCCUCUCGUCGGaGGAGc -3' miRNA: 3'- ucgUGAGAa--------CGGAGGGCAGCCgUCUC- -5' |
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| 28471 | 3' | -58.1 | NC_005946.1 | + | 33828 | 0.67 | 0.642891 |
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Target: 5'- uGGUcCUCaacGCCUCCuCGUUGGaCAGGGg -3' miRNA: 3'- -UCGuGAGaa-CGGAGG-GCAGCC-GUCUC- -5' |
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| 28471 | 3' | -58.1 | NC_005946.1 | + | 86622 | 0.72 | 0.364528 |
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Target: 5'- gGGCAUgaUCUggUGCCUUCCGcCGuGCAGGGa -3' miRNA: 3'- -UCGUG--AGA--ACGGAGGGCaGC-CGUCUC- -5' |
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| 28471 | 3' | -58.1 | NC_005946.1 | + | 37816 | 0.76 | 0.232709 |
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Target: 5'- cGGC-CUCUcUGCCgccauUCCCGUCaGGCAGAGc -3' miRNA: 3'- -UCGuGAGA-ACGG-----AGGGCAG-CCGUCUC- -5' |
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| 28471 | 3' | -58.1 | NC_005946.1 | + | 37366 | 0.82 | 0.088818 |
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Target: 5'- gGGCACUC--GCUUUCCGUCGGCGGGGu -3' miRNA: 3'- -UCGUGAGaaCGGAGGGCAGCCGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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