Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28472 | 5' | -59.6 | NC_005946.1 | + | 80959 | 0.66 | 0.679225 |
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Target: 5'- cCGuGCUAGCGgGAGCgaucaugcucaggGCUccGGCGUg -3' miRNA: 3'- aGC-CGGUCGCgCUCG-------------UGAuaCCGCGg -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 30082 | 0.66 | 0.670054 |
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Target: 5'- gUCGGCgAGgaccaCGUccuGCcggGCUAUGGCGCCc -3' miRNA: 3'- -AGCCGgUC-----GCGcu-CG---UGAUACCGCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 20755 | 0.66 | 0.649594 |
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Target: 5'- gUUGGCCAGaaaGAGgGCUccgauGUGGCGUUg -3' miRNA: 3'- -AGCCGGUCgcgCUCgUGA-----UACCGCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 33161 | 0.67 | 0.59834 |
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Target: 5'- -aGGCUGGCGUGguccGGCGCUGcGGgGUCc -3' miRNA: 3'- agCCGGUCGCGC----UCGUGAUaCCgCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 103240 | 0.67 | 0.567827 |
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Target: 5'- cUCGGCCGGCcccugcuacGcCGGGgGCUGcaGCGCCu -3' miRNA: 3'- -AGCCGGUCG---------C-GCUCgUGAUacCGCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 14287 | 0.68 | 0.557737 |
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Target: 5'- -aGGCCGGCGUacaGGCACUcu-GCGUCg -3' miRNA: 3'- agCCGGUCGCGc--UCGUGAuacCGCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 17569 | 0.69 | 0.498487 |
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Target: 5'- gCGGCCGG-GCaGGCACUGgaccuccucaGGCGCg -3' miRNA: 3'- aGCCGGUCgCGcUCGUGAUa---------CCGCGg -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 77290 | 0.69 | 0.498487 |
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Target: 5'- gUCGGCCGGaaaGAcGUACU-UGGCGUCg -3' miRNA: 3'- -AGCCGGUCgcgCU-CGUGAuACCGCGG- -5' |
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| 28472 | 5' | -59.6 | NC_005946.1 | + | 92923 | 0.71 | 0.349184 |
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Target: 5'- aCGGCUccagggAGUGCGAGUACgggagGuGCGCCu -3' miRNA: 3'- aGCCGG------UCGCGCUCGUGaua--C-CGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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