Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28473 | 5' | -58.6 | NC_005946.1 | + | 26902 | 0.66 | 0.703729 |
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Target: 5'- gGCGG-CCACGGagGCGGCGUACGc--- -3' miRNA: 3'- -UGCCgGGUGUUg-CGCCGCGUGUacag -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 37297 | 0.66 | 0.6833 |
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Target: 5'- -gGGuUCCACGACGUgGGCGCcUAUGUa -3' miRNA: 3'- ugCC-GGGUGUUGCG-CCGCGuGUACAg -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 69906 | 0.66 | 0.673016 |
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Target: 5'- gUGGCCUcCAGgGCcucGGCGUcCAUGUCa -3' miRNA: 3'- uGCCGGGuGUUgCG---CCGCGuGUACAG- -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 32315 | 0.67 | 0.646139 |
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Target: 5'- gACGGUCCaccaccagaGCAugGCGGCccuccucauggagcaGCACGcGUCc -3' miRNA: 3'- -UGCCGGG---------UGUugCGCCG---------------CGUGUaCAG- -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 45924 | 0.67 | 0.630589 |
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Target: 5'- -gGGCCCGCGuaaccguACGCGGCcCugG-GUCu -3' miRNA: 3'- ugCCGGGUGU-------UGCGCCGcGugUaCAG- -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 42192 | 0.68 | 0.579949 |
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Target: 5'- gUGGCCUcCAuCaCGGCGCACAUGg- -3' miRNA: 3'- uGCCGGGuGUuGcGCCGCGUGUACag -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 85488 | 0.69 | 0.499563 |
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Target: 5'- gAUGGCCCAgAugGCGGC-CGCGg--- -3' miRNA: 3'- -UGCCGGGUgUugCGCCGcGUGUacag -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 46026 | 0.71 | 0.424502 |
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Target: 5'- cCGGCCCACGACGCGGgauCGUaaccgGCggGUg -3' miRNA: 3'- uGCCGGGUGUUGCGCC---GCG-----UGuaCAg -5' |
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| 28473 | 5' | -58.6 | NC_005946.1 | + | 71977 | 0.74 | 0.269793 |
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Target: 5'- cACGuGCgCCACAGCGCGGCGUgauCGUGcCc -3' miRNA: 3'- -UGC-CG-GGUGUUGCGCCGCGu--GUACaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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