Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28482 | 3' | -58.8 | NC_005946.1 | + | 37187 | 0.66 | 0.675023 |
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Target: 5'- gGAgUGCCUGGAUCCaCgCgggugGAGUaGCACa -3' miRNA: 3'- -CUgAUGGACCUGGG-G-Ga----CUCGaCGUG- -5' |
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| 28482 | 3' | -58.8 | NC_005946.1 | + | 24183 | 0.66 | 0.664638 |
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Target: 5'- aGAcCUACCUGGACgcccuaCCCCUG-GCcGCcaGCa -3' miRNA: 3'- -CU-GAUGGACCUG------GGGGACuCGaCG--UG- -5' |
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| 28482 | 3' | -58.8 | NC_005946.1 | + | 31370 | 0.67 | 0.633354 |
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Target: 5'- uGACUACCgagGGuGCCuCCCUGAcgGCgGCGg -3' miRNA: 3'- -CUGAUGGa--CC-UGG-GGGACU--CGaCGUg -5' |
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| 28482 | 3' | -58.8 | NC_005946.1 | + | 26456 | 0.67 | 0.622912 |
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Target: 5'- ---gGCCgggUGGACgCUCCUGGGCUGgACc -3' miRNA: 3'- cugaUGG---ACCUG-GGGGACUCGACgUG- -5' |
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| 28482 | 3' | -58.8 | NC_005946.1 | + | 14197 | 0.67 | 0.599978 |
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Target: 5'- uACUGCCcgcacucUGGGuCCCCCaUGAGCcucagcgucacauUGCACu -3' miRNA: 3'- cUGAUGG-------ACCU-GGGGG-ACUCG-------------ACGUG- -5' |
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| 28482 | 3' | -58.8 | NC_005946.1 | + | 61196 | 0.68 | 0.550459 |
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Target: 5'- uGGCUAgggcCCUGGuggCCCUGAcuGCUGCACu -3' miRNA: 3'- -CUGAU----GGACCuggGGGACU--CGACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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