Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28482 | 5' | -60 | NC_005946.1 | + | 2982 | 0.66 | 0.628892 |
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Target: 5'- aUGGUaGga-GGGGCCGGCAGGUUUcaggggaGACa -3' miRNA: 3'- -ACCA-CaugCCCCGGUCGUUCGGG-------CUG- -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 92245 | 0.67 | 0.578619 |
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Target: 5'- aGGUGgggaucggcUGCGGaGGCCAGgAA-CCCGAg -3' miRNA: 3'- aCCAC---------AUGCC-CCGGUCgUUcGGGCUg -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 22029 | 0.67 | 0.558319 |
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Target: 5'- gGGUGaGgGGGGCCGuguGGCCCGuCu -3' miRNA: 3'- aCCACaUgCCCCGGUcguUCGGGCuG- -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 95203 | 0.68 | 0.527279 |
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Target: 5'- gUGGcUGUGCuGGgucugacGGCCAGCAGGUCCa-- -3' miRNA: 3'- -ACC-ACAUG-CC-------CCGGUCGUUCGGGcug -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 103034 | 0.68 | 0.518386 |
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Target: 5'- aGGacgccgGCuGGGCCAcUAGGCCCGACg -3' miRNA: 3'- aCCaca---UGcCCCGGUcGUUCGGGCUG- -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 93541 | 0.68 | 0.518386 |
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Target: 5'- aGGUGUACGacGGCC-GUAGGuggaaCCCGGCg -3' miRNA: 3'- aCCACAUGCc-CCGGuCGUUC-----GGGCUG- -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 62073 | 0.69 | 0.427908 |
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Target: 5'- aUGGUGU-CGGGgacguagaggagaaaGCaagagaugGGCAGGCCCGACc -3' miRNA: 3'- -ACCACAuGCCC---------------CGg-------UCGUUCGGGCUG- -5' |
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| 28482 | 5' | -60 | NC_005946.1 | + | 29583 | 0.76 | 0.156706 |
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Target: 5'- gUGGgagGUAUGGGGCCAGaGGGCCCa-- -3' miRNA: 3'- -ACCa--CAUGCCCCGGUCgUUCGGGcug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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