Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28487 | 5' | -56 | NC_005946.1 | + | 50267 | 0.66 | 0.851782 |
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Target: 5'- uGAGGAGcCCGuGAGGGUAccccguaGUCGUUg -3' miRNA: 3'- -CUCCUCuGGCuUUCCCGUc------CAGCAGu -5' |
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| 28487 | 5' | -56 | NC_005946.1 | + | 60189 | 0.66 | 0.826639 |
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Target: 5'- -uGGAaGCCGAGuuucGGGCAGGUC-UCc -3' miRNA: 3'- cuCCUcUGGCUUu---CCCGUCCAGcAGu -5' |
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| 28487 | 5' | -56 | NC_005946.1 | + | 91264 | 0.66 | 0.82577 |
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Target: 5'- gGGGGAGGCCGAcAAGGGCAcccugcuGGaCGc-- -3' miRNA: 3'- -CUCCUCUGGCU-UUCCCGU-------CCaGCagu -5' |
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| 28487 | 5' | -56 | NC_005946.1 | + | 4696 | 0.66 | 0.817874 |
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Target: 5'- cGAGcGAGACUGcuacaAGAGGGCGGccUGUCAg -3' miRNA: 3'- -CUC-CUCUGGC-----UUUCCCGUCcaGCAGU- -5' |
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| 28487 | 5' | -56 | NC_005946.1 | + | 30860 | 0.68 | 0.752064 |
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Target: 5'- gGAGGGGACCacGAccGGGUgagcggccgccAGGUCGUa- -3' miRNA: 3'- -CUCCUCUGG--CUuuCCCG-----------UCCAGCAgu -5' |
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| 28487 | 5' | -56 | NC_005946.1 | + | 86229 | 0.82 | 0.128996 |
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Target: 5'- aGGGAGGCCGAGAGGGCcucGGGUCaGUUg -3' miRNA: 3'- cUCCUCUGGCUUUCCCG---UCCAG-CAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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