Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28489 | 5' | -57.6 | NC_005946.1 | + | 17723 | 0.67 | 0.754517 |
Target: 5'- cGGGaGUcGUGG--AGGGUA-GUGGGGg -3' miRNA: 3'- uCCCaCA-CACCcuUCCCAUgCACCCC- -5' |
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28489 | 5' | -57.6 | NC_005946.1 | + | 101614 | 0.67 | 0.715196 |
Target: 5'- cAGGGUGgGUGuGGAAGGacacgaGgcCGUcGGGGg -3' miRNA: 3'- -UCCCACaCAC-CCUUCC------CauGCA-CCCC- -5' |
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28489 | 5' | -57.6 | NC_005946.1 | + | 47955 | 0.69 | 0.623414 |
Target: 5'- --------cGGGAGGGuGUACGUGGGGa -3' miRNA: 3'- ucccacacaCCCUUCC-CAUGCACCCC- -5' |
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28489 | 5' | -57.6 | NC_005946.1 | + | 2843 | 0.73 | 0.410234 |
Target: 5'- aAGGGUGgaGUGGGAgccAGGGggcuaGUGGGa -3' miRNA: 3'- -UCCCACa-CACCCU---UCCCaug--CACCCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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