Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28510 | 3' | -58.8 | NC_005946.1 | + | 43766 | 0.71 | 0.436237 |
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Target: 5'- gCCUCCCGcUCacgGCGcagccagggagaGGGGCUCCGUCUc -3' miRNA: 3'- aGGAGGGC-AGa--UGC------------UCCUGGGGCAGG- -5' |
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| 28510 | 3' | -58.8 | NC_005946.1 | + | 41957 | 0.72 | 0.43093 |
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Target: 5'- cUCUgaCCCGUCUGCGAuGGCCCucagccucugcacugCGUCCa -3' miRNA: 3'- -AGGa-GGGCAGAUGCUcCUGGG---------------GCAGG- -5' |
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| 28510 | 3' | -58.8 | NC_005946.1 | + | 42406 | 0.72 | 0.401588 |
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Target: 5'- gCCUCCCGUUgACGAcGuGACCCaCGUgCa -3' miRNA: 3'- aGGAGGGCAGaUGCU-C-CUGGG-GCAgG- -5' |
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| 28510 | 3' | -58.8 | NC_005946.1 | + | 83380 | 0.73 | 0.376777 |
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Target: 5'- gCCUCCCGuagucggcgcacUCUGCcucGAGGGCCCUGUa- -3' miRNA: 3'- aGGAGGGC------------AGAUG---CUCCUGGGGCAgg -5' |
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| 28510 | 3' | -58.8 | NC_005946.1 | + | 76381 | 0.81 | 0.112824 |
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Target: 5'- uUUCUCCCagagaCUAUGAGGACCUCGUCCu -3' miRNA: 3'- -AGGAGGGca---GAUGCUCCUGGGGCAGG- -5' |
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| 28510 | 3' | -58.8 | NC_005946.1 | + | 70110 | 0.88 | 0.040172 |
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Target: 5'- uUCCUCCCGUCUcccuUGAGGACCCUGaUCCu -3' miRNA: 3'- -AGGAGGGCAGAu---GCUCCUGGGGC-AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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