Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28512 | 5' | -58.5 | NC_005946.1 | + | 27182 | 0.66 | 0.692484 |
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Target: 5'- ------gGUCUCCGCCuacgGGCCCUaCUCg -3' miRNA: 3'- gaagauaCAGGGGCGG----CCGGGA-GAGa -5' |
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| 28512 | 5' | -58.5 | NC_005946.1 | + | 3325 | 0.67 | 0.686363 |
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Target: 5'- -cUCcAUGUCCCCGCCagagaguaucucccuGGUCCUgaCUg -3' miRNA: 3'- gaAGaUACAGGGGCGG---------------CCGGGAgaGA- -5' |
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| 28512 | 5' | -58.5 | NC_005946.1 | + | 58476 | 0.67 | 0.659677 |
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Target: 5'- cCUUCggcgugagcgGUCUgCGCCGGCCaCUCUg- -3' miRNA: 3'- -GAAGaua-------CAGGgGCGGCCGG-GAGAga -5' |
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| 28512 | 5' | -58.5 | NC_005946.1 | + | 22075 | 0.68 | 0.610117 |
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Target: 5'- -gUCUGUGUUCUCGa-GGCCCUUUUUc -3' miRNA: 3'- gaAGAUACAGGGGCggCCGGGAGAGA- -5' |
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| 28512 | 5' | -58.5 | NC_005946.1 | + | 10161 | 0.7 | 0.470417 |
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Target: 5'- aUUCUcgGUcagguauccucCCCUGauGGCCCUCUCUg -3' miRNA: 3'- gAAGAuaCA-----------GGGGCggCCGGGAGAGA- -5' |
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| 28512 | 5' | -58.5 | NC_005946.1 | + | 33441 | 0.71 | 0.424421 |
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Target: 5'- ------cGUCCCCGUCGGaCUCUCUCc -3' miRNA: 3'- gaagauaCAGGGGCGGCC-GGGAGAGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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