Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28513 | 5' | -56.9 | NC_005946.1 | + | 66070 | 0.66 | 0.801396 |
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Target: 5'- --aGGGCUCUGGcuauggccgcGGCUGCggCGGCCu -3' miRNA: 3'- gagUCUGAGACU----------CUGACGgaGCCGGu -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 58279 | 0.66 | 0.78277 |
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Target: 5'- -cCAGGCUCaGGGACucUGCCacccUGGCCGc -3' miRNA: 3'- gaGUCUGAGaCUCUG--ACGGa---GCCGGU- -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 83720 | 0.66 | 0.773239 |
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Target: 5'- cCUCGGGCU----GGCUGCCcUGGCCGc -3' miRNA: 3'- -GAGUCUGAgacuCUGACGGaGCCGGU- -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 51453 | 0.72 | 0.44876 |
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Target: 5'- gUCAGACUCUGAGGCUgaaGCCccCGuaccuGCCAu -3' miRNA: 3'- gAGUCUGAGACUCUGA---CGGa-GC-----CGGU- -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 52596 | 0.72 | 0.44876 |
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Target: 5'- uCUCAGAgUCUGAGACgUGCg-CGGCa- -3' miRNA: 3'- -GAGUCUgAGACUCUG-ACGgaGCCGgu -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 85118 | 0.74 | 0.34488 |
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Target: 5'- -aCAGGCUCcugGAGcagGCCUCGGCCGu -3' miRNA: 3'- gaGUCUGAGa--CUCugaCGGAGCCGGU- -5' |
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| 28513 | 5' | -56.9 | NC_005946.1 | + | 25435 | 0.76 | 0.271715 |
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Target: 5'- -cCAGACcCUGAGGCUGUCUCccacagaGGCCAu -3' miRNA: 3'- gaGUCUGaGACUCUGACGGAG-------CCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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