Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28515 | 3' | -58.2 | NC_005946.1 | + | 11203 | 0.66 | 0.733299 |
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Target: 5'- aCGGGGGCC-CUcagaggaggauUGGCGUCGAGgaGGUg -3' miRNA: 3'- -GUUCCUGGaGG-----------ACCGUAGCUCg-CCGu -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 59925 | 0.66 | 0.733299 |
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Target: 5'- gAAGGGCaC-CUUGGCGUCGGGCcgauugccGGUg -3' miRNA: 3'- gUUCCUG-GaGGACCGUAGCUCG--------CCGu -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 6291 | 0.66 | 0.723278 |
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Target: 5'- --uGGGCUgcucuccCCUGGCGUCGAGCa--- -3' miRNA: 3'- guuCCUGGa------GGACCGUAGCUCGccgu -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 33124 | 0.66 | 0.713176 |
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Target: 5'- aAAGGGCCUgCCgGGCuucuUUGAG-GGCGc -3' miRNA: 3'- gUUCCUGGA-GGaCCGu---AGCUCgCCGU- -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 44655 | 0.67 | 0.672148 |
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Target: 5'- aGAGGACCggCCUGuGCcu--GGCGGCGg -3' miRNA: 3'- gUUCCUGGa-GGAC-CGuagcUCGCCGU- -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 61106 | 0.67 | 0.651394 |
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Target: 5'- gGAGGGCgCUgUUGGCGUUGGG-GGCc -3' miRNA: 3'- gUUCCUG-GAgGACCGUAGCUCgCCGu -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 3527 | 0.67 | 0.639948 |
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Target: 5'- cCGAGGGCCUCCuUGGacuuugaCGUUGAcaugGCGGUg -3' miRNA: 3'- -GUUCCUGGAGG-ACC-------GUAGCU----CGCCGu -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 68279 | 0.69 | 0.558166 |
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Target: 5'- cCAGGGACCUCaacagGGCGg-GAGCGucGCAg -3' miRNA: 3'- -GUUCCUGGAGga---CCGUagCUCGC--CGU- -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 2043 | 0.74 | 0.265447 |
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Target: 5'- cCGAGGACCUCCgccaucucacgcaGGCgaccGUCGAggGCGGCAg -3' miRNA: 3'- -GUUCCUGGAGGa------------CCG----UAGCU--CGCCGU- -5' |
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| 28515 | 3' | -58.2 | NC_005946.1 | + | 17585 | 0.76 | 0.209499 |
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Target: 5'- --uGGACCUCCUcaGGCG-CGAGgGGCAc -3' miRNA: 3'- guuCCUGGAGGA--CCGUaGCUCgCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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