Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28515 | 5' | -58.8 | NC_005946.1 | + | 103480 | 0.67 | 0.672884 |
Target: 5'- --cGCGGaccugauaGACAcCCAGGCccaGGUGGaCCa -3' miRNA: 3'- acaUGCCg-------CUGU-GGUCCGa--CCACC-GG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 92730 | 0.78 | 0.153628 |
Target: 5'- --gGCGGUGGCAgucaccguccaCAGGCUGGUGGCUg -3' miRNA: 3'- acaUGCCGCUGUg----------GUCCGACCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 92572 | 0.68 | 0.570352 |
Target: 5'- cUGcUGCuGGgGu--CCAGGgUGGUGGCCg -3' miRNA: 3'- -AC-AUG-CCgCuguGGUCCgACCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 92315 | 0.66 | 0.703365 |
Target: 5'- aGUACaGCguaaaguacGACuACCuGGUgagGGUGGCCu -3' miRNA: 3'- aCAUGcCG---------CUG-UGGuCCGa--CCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 89687 | 0.67 | 0.63181 |
Target: 5'- aGgagACGGUGGCACCGuccGCcaUGGaGGCCg -3' miRNA: 3'- aCa--UGCCGCUGUGGUc--CG--ACCaCCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 85009 | 0.66 | 0.692239 |
Target: 5'- cUGU-CGGCcucaGACGCCcuggagcAGGCcgccgcGGUGGCCc -3' miRNA: 3'- -ACAuGCCG----CUGUGG-------UCCGa-----CCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 84773 | 0.7 | 0.481481 |
Target: 5'- cUGUcugACaGCGugAUCAGGCUGGgcaGGUCc -3' miRNA: 3'- -ACA---UGcCGCugUGGUCCGACCa--CCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 83708 | 0.77 | 0.19309 |
Target: 5'- --aGCGGCGGCuACCcucGGGCUGGcugcccUGGCCg -3' miRNA: 3'- acaUGCCGCUG-UGG---UCCGACC------ACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 49764 | 0.74 | 0.291895 |
Target: 5'- cGgggAUGGCGACugC-GGCU-GUGGCCg -3' miRNA: 3'- aCa--UGCCGCUGugGuCCGAcCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 46190 | 0.66 | 0.723398 |
Target: 5'- --cACGGUGGCgGCC-GGCUGGgcaagGGUa -3' miRNA: 3'- acaUGCCGCUG-UGGuCCGACCa----CCGg -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 42168 | 0.74 | 0.291895 |
Target: 5'- cUGga-GGCGACGCUgucuGGGaUGGUGGCCu -3' miRNA: 3'- -ACaugCCGCUGUGG----UCCgACCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 38187 | 0.68 | 0.590735 |
Target: 5'- --cACGGUGACcUCGGGCUGaGggacgucgGGCCc -3' miRNA: 3'- acaUGCCGCUGuGGUCCGAC-Ca-------CCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 33760 | 0.66 | 0.693253 |
Target: 5'- --gAUGGCc-CACgGGGCccugccggUGGUGGCCa -3' miRNA: 3'- acaUGCCGcuGUGgUCCG--------ACCACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 33215 | 0.66 | 0.703365 |
Target: 5'- cUGUGCGGCGugA-CGGGUgaccccguggGGUucgugagggaGGCCa -3' miRNA: 3'- -ACAUGCCGCugUgGUCCGa---------CCA----------CCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 33146 | 0.7 | 0.462618 |
Target: 5'- --gAgGGCGcuccCGCCAGGCUGGcgUGGUCc -3' miRNA: 3'- acaUgCCGCu---GUGGUCCGACC--ACCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 33099 | 0.7 | 0.472002 |
Target: 5'- uUGUGCuGGC--CACCgucAGGCUGGgaaagGGCCu -3' miRNA: 3'- -ACAUG-CCGcuGUGG---UCCGACCa----CCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 28653 | 0.66 | 0.693253 |
Target: 5'- aGUGCGGgccaGuCugCGGGCUGGa-GCCc -3' miRNA: 3'- aCAUGCCg---CuGugGUCCGACCacCGG- -5' |
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28515 | 5' | -58.8 | NC_005946.1 | + | 26910 | 0.75 | 0.229676 |
Target: 5'- cGgagGCGGCGuACGCCAgGGCcGGgGGCCa -3' miRNA: 3'- aCa--UGCCGC-UGUGGU-CCGaCCaCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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