Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28516 | 3' | -59.1 | NC_005946.1 | + | 29550 | 0.66 | 0.722894 |
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Target: 5'- gGGGCgCCUGGAuCUCagGGCCcggACGGAg -3' miRNA: 3'- -CCCGaGGACCU-GAG--CCGGcagUGUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 55071 | 0.66 | 0.722894 |
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Target: 5'- aGGGCcucaaCCacGAUUUGGCCG-CACAGGa -3' miRNA: 3'- -CCCGa----GGacCUGAGCCGGCaGUGUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 86364 | 0.66 | 0.722894 |
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Target: 5'- uGGGCUCagaCUGuGACUUGcGCUGguauaGCAGAg -3' miRNA: 3'- -CCCGAG---GAC-CUGAGC-CGGCag---UGUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 32500 | 0.66 | 0.703007 |
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Target: 5'- aGGGCUCCaaggaGGACUacgucaGGCaCGUCcuggcCGGAg -3' miRNA: 3'- -CCCGAGGa----CCUGAg-----CCG-GCAGu----GUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 96183 | 0.68 | 0.581092 |
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Target: 5'- uGGGC-CCUGGACaugaCGGCCauccuguuGUCGgGGGu -3' miRNA: 3'- -CCCGaGGACCUGa---GCCGG--------CAGUgUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 11899 | 0.69 | 0.540981 |
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Target: 5'- aGGGC-CCgcagGGACgcccCGGCCGUC-CuGAc -3' miRNA: 3'- -CCCGaGGa---CCUGa---GCCGGCAGuGuCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 95441 | 0.7 | 0.445631 |
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Target: 5'- uGGCUCCuaUGGACUgGGCg--CACAGGa -3' miRNA: 3'- cCCGAGG--ACCUGAgCCGgcaGUGUCU- -5' |
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| 28516 | 3' | -59.1 | NC_005946.1 | + | 85120 | 0.75 | 0.237516 |
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Target: 5'- aGGCUCCUGGAgcaggcCUCGGCCGUguCcGGc -3' miRNA: 3'- cCCGAGGACCU------GAGCCGGCAguGuCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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