Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28524 | 3' | -56.8 | NC_005946.1 | + | 22028 | 0.76 | 0.285473 |
Target: 5'- aGGGUGAGGGGGGcCGUGuGGCCcgUCUa -3' miRNA: 3'- -UCCGCUUCCCCU-GCAU-CCGGaaAGGg -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 74608 | 0.73 | 0.428153 |
Target: 5'- -cGUGAGGagaGGGACGUAGGUCUccgCCCu -3' miRNA: 3'- ucCGCUUC---CCCUGCAUCCGGAaa-GGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 38380 | 0.71 | 0.539558 |
Target: 5'- cAGGCGAAGccggccuugaccaGGACGUcugAGGCCUUUCg- -3' miRNA: 3'- -UCCGCUUCc------------CCUGCA---UCCGGAAAGgg -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 49032 | 0.7 | 0.563937 |
Target: 5'- gGGGCGAAGaGGACGgGGGCCcggaUCUa -3' miRNA: 3'- -UCCGCUUCcCCUGCaUCCGGaa--AGGg -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 13321 | 0.69 | 0.615521 |
Target: 5'- uGGCGucgacGGGGACGUuuacCUUUUCCCa -3' miRNA: 3'- uCCGCuu---CCCCUGCAucc-GGAAAGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 37290 | 0.69 | 0.615521 |
Target: 5'- cAGGCGAGGGuuccacGACGUGGGCg---CCUa -3' miRNA: 3'- -UCCGCUUCCc-----CUGCAUCCGgaaaGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 6218 | 0.69 | 0.636298 |
Target: 5'- cGGCacAGGacuucGAUGUGGGCCUcUCCCu -3' miRNA: 3'- uCCGcuUCCc----CUGCAUCCGGAaAGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 38198 | 0.68 | 0.704384 |
Target: 5'- cGGGCuGAG-GGACGUcGGGCCcccucucgaccugUUCCCa -3' miRNA: 3'- -UCCGcUUCcCCUGCA-UCCGGa------------AAGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 33057 | 0.68 | 0.688028 |
Target: 5'- uGGCcGAGGGGGCG-GGGUUc-UCCCu -3' miRNA: 3'- uCCGcUUCCCCUGCaUCCGGaaAGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 32023 | 0.68 | 0.677742 |
Target: 5'- aGGGaaaGGAGaGGGugGUGGuGCCUcacaucaggCCCg -3' miRNA: 3'- -UCCg--CUUC-CCCugCAUC-CGGAaa-------GGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 93156 | 0.67 | 0.724598 |
Target: 5'- aGGGCGGAGGGaGGC--AGGCUggagcugucugcUCCCu -3' miRNA: 3'- -UCCGCUUCCC-CUGcaUCCGGaa----------AGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 46252 | 0.67 | 0.728604 |
Target: 5'- cGGGCcu-GGGGACGUagcccaGGGCCaccgcUCCUc -3' miRNA: 3'- -UCCGcuuCCCCUGCA------UCCGGaa---AGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 88110 | 0.67 | 0.742512 |
Target: 5'- -cGCuGGAGGGGAUGUaaaugcuuacucuguGuGGCCUUaCCCg -3' miRNA: 3'- ucCG-CUUCCCCUGCA---------------U-CCGGAAaGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 61276 | 0.67 | 0.758167 |
Target: 5'- gAGuCGAAGGGGAuccugugcuucUGUAGGuaCCUggCCCa -3' miRNA: 3'- -UCcGCUUCCCCU-----------GCAUCC--GGAaaGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 58164 | 0.67 | 0.764925 |
Target: 5'- gAGGCGucagggucacucacGAGGagguccgugaGGACGUAGGgCCUgaaccucaUCCCg -3' miRNA: 3'- -UCCGC--------------UUCC----------CCUGCAUCC-GGAa-------AGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 22904 | 0.66 | 0.805015 |
Target: 5'- cGGCGAGGGGaGuccACGgcaugGGGuCCggagCCCa -3' miRNA: 3'- uCCGCUUCCC-C---UGCa----UCC-GGaaa-GGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 10540 | 0.66 | 0.805015 |
Target: 5'- cAGGCccAGGGGcuuCGggggAGGCaggUUCCCc -3' miRNA: 3'- -UCCGcuUCCCCu--GCa---UCCGga-AAGGG- -5' |
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28524 | 3' | -56.8 | NC_005946.1 | + | 58012 | 0.66 | 0.822688 |
Target: 5'- uGGCccucacccucAGGGaGAUGUAGGCCUcgCCg -3' miRNA: 3'- uCCGcu--------UCCC-CUGCAUCCGGAaaGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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