Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28526 | 5' | -58.9 | NC_005946.1 | + | 89306 | 0.66 | 0.709437 |
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Target: 5'- cACAUGGCaaacagGGUCauGGGCUcgUCGGCGg -3' miRNA: 3'- -UGUGCCGga----CCAGccUCCGA--AGCUGC- -5' |
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| 28526 | 5' | -58.9 | NC_005946.1 | + | 48932 | 0.67 | 0.658894 |
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Target: 5'- uCACGGguccCCUGGUCcagucggauGGAGGCgucaUGACGg -3' miRNA: 3'- uGUGCC----GGACCAG---------CCUCCGaa--GCUGC- -5' |
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| 28526 | 5' | -58.9 | NC_005946.1 | + | 83030 | 0.68 | 0.597612 |
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Target: 5'- cCACGGCagacaacaucaUGGcCGGAGGCa-CGGCGa -3' miRNA: 3'- uGUGCCGg----------ACCaGCCUCCGaaGCUGC- -5' |
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| 28526 | 5' | -58.9 | NC_005946.1 | + | 2091 | 0.69 | 0.557159 |
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Target: 5'- uGCGCGGCUgacGUCGGAGaGCUaaaGAUGg -3' miRNA: 3'- -UGUGCCGGac-CAGCCUC-CGAag-CUGC- -5' |
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| 28526 | 5' | -58.9 | NC_005946.1 | + | 102212 | 0.71 | 0.424179 |
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Target: 5'- aGCuCGGCCUcGUCGGGGGCgUCuGCGu -3' miRNA: 3'- -UGuGCCGGAcCAGCCUCCGaAGcUGC- -5' |
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| 28526 | 5' | -58.9 | NC_005946.1 | + | 88225 | 0.71 | 0.424179 |
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Target: 5'- cGCGCaGUCUGcGcUCGGAGGCUUC-ACGg -3' miRNA: 3'- -UGUGcCGGAC-C-AGCCUCCGAAGcUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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