Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28527 | 3' | -58.6 | NC_005946.1 | + | 8000 | 0.66 | 0.686419 |
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Target: 5'- cUACCAGCCUCAcGaGcGGCCUcugggcCCCGCa- -3' miRNA: 3'- -GUGGUCGGAGU-C-C-CUGGAa-----GGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 22652 | 0.66 | 0.665619 |
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Target: 5'- gGCCAGagaCCUucucgCAGGaGACCUUCUCGCa- -3' miRNA: 3'- gUGGUC---GGA-----GUCC-CUGGAAGGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 83081 | 0.66 | 0.644705 |
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Target: 5'- aCGCCGGgUUCAGGGACCagUUCUCu--- -3' miRNA: 3'- -GUGGUCgGAGUCCCUGG--AAGGGugaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 4272 | 0.66 | 0.644705 |
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Target: 5'- aCACgaGGCCgUCGGGGGC--UCCCACg- -3' miRNA: 3'- -GUGg-UCGG-AGUCCCUGgaAGGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 79066 | 0.67 | 0.602819 |
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Target: 5'- uGgCGGCCUCGGucauGGCCUUUCCGCa- -3' miRNA: 3'- gUgGUCGGAGUCc---CUGGAAGGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 47626 | 0.67 | 0.581981 |
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Target: 5'- -uCCAGgCUCAGGGACUcUgCCACc- -3' miRNA: 3'- guGGUCgGAGUCCCUGGaAgGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 61852 | 0.68 | 0.540811 |
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Target: 5'- aCGCCcuGCC-CAGGGACCUguaugugacgggUCCgGCUUc -3' miRNA: 3'- -GUGGu-CGGaGUCCCUGGA------------AGGgUGAA- -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 11078 | 0.7 | 0.406862 |
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Target: 5'- cCGCCAGCCcCAGGGuCUcguacacgUCCCACa- -3' miRNA: 3'- -GUGGUCGGaGUCCCuGGa-------AGGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 102015 | 0.71 | 0.380935 |
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Target: 5'- uGCCAG-CUCGGGGGCCUgCUCGCc- -3' miRNA: 3'- gUGGUCgGAGUCCCUGGAaGGGUGaa -5' |
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| 28527 | 3' | -58.6 | NC_005946.1 | + | 80376 | 0.71 | 0.367565 |
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Target: 5'- gACCAGCCUgucccugccuucggaCAGGGGCCcUCCgACg- -3' miRNA: 3'- gUGGUCGGA---------------GUCCCUGGaAGGgUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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