Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28529 | 3' | -54 | NC_005946.1 | + | 75104 | 0.66 | 0.916106 |
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Target: 5'- gCCguagagGCCCGGGGGCAucuuaacgGcCAGGUCu -3' miRNA: 3'- -GGagaaa-UGGGUCCCCGU--------C-GUCUAGu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 2294 | 0.66 | 0.916106 |
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Target: 5'- uCCUCggacgcggACCCcgccGGGGGCugguGCAGAa-- -3' miRNA: 3'- -GGAGaaa-----UGGG----UCCCCGu---CGUCUagu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 2630 | 0.66 | 0.889904 |
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Target: 5'- gCCUUcacACCCAGGGGCAccuGCGGc--- -3' miRNA: 3'- -GGAGaaaUGGGUCCCCGU---CGUCuagu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 13143 | 0.67 | 0.875307 |
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Target: 5'- --gCUguuCCCAGGGGCAGggacacaccCAGGUCc -3' miRNA: 3'- ggaGAaauGGGUCCCCGUC---------GUCUAGu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 63178 | 0.67 | 0.843323 |
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Target: 5'- -----cUGCCCAGGGGUuugcagucgAGCAGcUCAa -3' miRNA: 3'- ggagaaAUGGGUCCCCG---------UCGUCuAGU- -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 76652 | 0.67 | 0.842478 |
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Target: 5'- aCCaggg-GCCCAGGGGCccaggggAGCAGAg-- -3' miRNA: 3'- -GGagaaaUGGGUCCCCG-------UCGUCUagu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 63665 | 0.69 | 0.760026 |
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Target: 5'- cCCUCccUugCUAGGGGC-GCAGAc-- -3' miRNA: 3'- -GGAGaaAugGGUCCCCGuCGUCUagu -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 88589 | 0.7 | 0.719364 |
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Target: 5'- aCUCcauagacgGCCUGGGGGCAGUuguggccuacaAGAUCAu -3' miRNA: 3'- gGAGaaa-----UGGGUCCCCGUCG-----------UCUAGU- -5' |
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| 28529 | 3' | -54 | NC_005946.1 | + | 95369 | 0.71 | 0.634779 |
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Target: 5'- aCCgUCag-GCCCAGGGGCuucgggggaGGCAGGUUc -3' miRNA: 3'- -GG-AGaaaUGGGUCCCCG---------UCGUCUAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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