Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28531 | 3' | -56.9 | NC_005946.1 | + | 48607 | 0.66 | 0.772838 |
Target: 5'- -cUCCucGGCcuGCUCCUcGGcaGUCAGGGACc -3' miRNA: 3'- cuAGGu-CCG--UGAGGA-CC--CAGUCUCUG- -5' |
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28531 | 3' | -56.9 | NC_005946.1 | + | 73384 | 0.67 | 0.712929 |
Target: 5'- --gUCAGGCACgUCCUGGc-CGGAGAa -3' miRNA: 3'- cuaGGUCCGUG-AGGACCcaGUCUCUg -5' |
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28531 | 3' | -56.9 | NC_005946.1 | + | 86562 | 0.67 | 0.711902 |
Target: 5'- --gCCAGcGCGCUCCUcucgagcuugaugGGGUUGGAGcCg -3' miRNA: 3'- cuaGGUC-CGUGAGGA-------------CCCAGUCUCuG- -5' |
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28531 | 3' | -56.9 | NC_005946.1 | + | 21177 | 0.68 | 0.660902 |
Target: 5'- --gUCGGGCuucaagagGCUCCUGGGaaagacggCAGAGAUa -3' miRNA: 3'- cuaGGUCCG--------UGAGGACCCa-------GUCUCUG- -5' |
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28531 | 3' | -56.9 | NC_005946.1 | + | 102056 | 0.69 | 0.612461 |
Target: 5'- gGGUCCAGGC-C-CCUGGucuugacgagccucUCGGAGACg -3' miRNA: 3'- -CUAGGUCCGuGaGGACCc-------------AGUCUCUG- -5' |
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28531 | 3' | -56.9 | NC_005946.1 | + | 75312 | 0.72 | 0.410452 |
Target: 5'- aGUCCAGGCugUCCaccuccagaaUGGGa-GGAGACu -3' miRNA: 3'- cUAGGUCCGugAGG----------ACCCagUCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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