Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28533 | 3' | -60.9 | NC_005946.1 | + | 35922 | 0.66 | 0.584333 |
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Target: 5'- uCGCa-GCGGGaCguuuggaagUCCUGGCCgUCCUGa -3' miRNA: 3'- cGCGgaCGUCC-G---------AGGACCGGaAGGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 8249 | 0.66 | 0.584333 |
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Target: 5'- --uCCUGCAuGGCgccgUCUGGCCacugCCUGg -3' miRNA: 3'- cgcGGACGU-CCGa---GGACCGGaa--GGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 70017 | 0.66 | 0.574159 |
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Target: 5'- -aGCCUcucgGaCAGGC-CCUGGCC--CCUGa -3' miRNA: 3'- cgCGGA----C-GUCCGaGGACCGGaaGGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 61201 | 0.66 | 0.549923 |
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Target: 5'- aGUGCCUggGCAGGCUgagGGCCaacggggggaccgUCCUGg -3' miRNA: 3'- -CGCGGA--CGUCCGAggaCCGGa------------AGGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 68992 | 0.66 | 0.543911 |
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Target: 5'- -aGCCUGCAGGCgcaCC-GGUCUuugUCCc- -3' miRNA: 3'- cgCGGACGUCCGa--GGaCCGGA---AGGac -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 67379 | 0.67 | 0.524036 |
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Target: 5'- -aGuCCUGCAgGGC-CCUGGUCacggCCUGa -3' miRNA: 3'- cgC-GGACGU-CCGaGGACCGGaa--GGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 61258 | 0.67 | 0.494768 |
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Target: 5'- aCGCCUGCgAGaGgaCC-GGCCUgugCCUGg -3' miRNA: 3'- cGCGGACG-UC-CgaGGaCCGGAa--GGAC- -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 48257 | 0.68 | 0.438638 |
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Target: 5'- gGUGCUUGCAGGCguaggUgaGGCgUUCCg- -3' miRNA: 3'- -CGCGGACGUCCGa----GgaCCGgAAGGac -5' |
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| 28533 | 3' | -60.9 | NC_005946.1 | + | 34590 | 0.69 | 0.395609 |
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Target: 5'- cGCGCCcgUGCuGGaUCCccugaagacggccagGGCCUUCCUGu -3' miRNA: 3'- -CGCGG--ACGuCCgAGGa--------------CCGGAAGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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