Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28541 | 5' | -57 | NC_005946.1 | + | 95217 | 0.66 | 0.757511 |
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Target: 5'- cAGGGgCCUGuacaAGACuguGCGUCACa-- -3' miRNA: 3'- -UCCCgGGACua--UCUGu--CGCAGUGguu -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 56148 | 0.66 | 0.747547 |
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Target: 5'- uGGGGCCUcgggGAUGGcgacuGCGGCuGUgGCCGAa -3' miRNA: 3'- -UCCCGGGa---CUAUC-----UGUCG-CAgUGGUU- -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 62078 | 0.67 | 0.685838 |
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Target: 5'- aGGGGUCCUGAaggAGGCcuggGGCcacGUCACCu- -3' miRNA: 3'- -UCCCGGGACUa--UCUG----UCG---CAGUGGuu -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 102485 | 0.67 | 0.675328 |
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Target: 5'- uGGGCCUUGAUGagcuuGAgGGCcGUCACUg- -3' miRNA: 3'- uCCCGGGACUAU-----CUgUCG-CAGUGGuu -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 66343 | 0.68 | 0.658436 |
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Target: 5'- cGaGGCCCUGAgggAGACcccguacgucucuucGGCGUgCGCCGc -3' miRNA: 3'- uC-CCGGGACUa--UCUG---------------UCGCA-GUGGUu -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 26968 | 0.69 | 0.548827 |
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Target: 5'- uGGGCCUUGGUGGcguCAGUGUU-CCAGg -3' miRNA: 3'- uCCCGGGACUAUCu--GUCGCAGuGGUU- -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 40196 | 0.7 | 0.538504 |
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Target: 5'- uGGGCCCUG-UGGAUcGUGUCGUCAAu -3' miRNA: 3'- uCCCGGGACuAUCUGuCGCAGUGGUU- -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 49431 | 0.72 | 0.377275 |
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Target: 5'- cAGGGCCCUGGaggGGACGGgGUCcUCGu -3' miRNA: 3'- -UCCCGGGACUa--UCUGUCgCAGuGGUu -5' |
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| 28541 | 5' | -57 | NC_005946.1 | + | 40754 | 0.74 | 0.298113 |
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Target: 5'- uGGGCCaccaagaCUGGUAGGCGGCGgCGCCGc -3' miRNA: 3'- uCCCGG-------GACUAUCUGUCGCaGUGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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