Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28545 | 3' | -52.8 | NC_005946.1 | + | 34664 | 0.66 | 0.942839 |
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Target: 5'- aCGUCCGCcaGGUCGGGCcUGGAcauggauAUCUg -3' miRNA: 3'- aGCAGGUG--UCAGUCCGcGCUU-------UAGAg -5' |
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| 28545 | 3' | -52.8 | NC_005946.1 | + | 24968 | 0.67 | 0.91449 |
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Target: 5'- cUUGUUUACGGUCAGGCcgccucccgugcuaGCGGGAgcgaucaugCUCa -3' miRNA: 3'- -AGCAGGUGUCAGUCCG--------------CGCUUUa--------GAG- -5' |
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| 28545 | 3' | -52.8 | NC_005946.1 | + | 76372 | 0.68 | 0.890029 |
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Target: 5'- -gGUCCAacCGGUCccagcgGGGCGCcuGGAUCUCa -3' miRNA: 3'- agCAGGU--GUCAG------UCCGCGc-UUUAGAG- -5' |
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| 28545 | 3' | -52.8 | NC_005946.1 | + | 26318 | 0.68 | 0.882824 |
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Target: 5'- cUUGUCCACcacgucaaAGUCcaGGGUgaGUGGAGUCUCu -3' miRNA: 3'- -AGCAGGUG--------UCAG--UCCG--CGCUUUAGAG- -5' |
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| 28545 | 3' | -52.8 | NC_005946.1 | + | 9336 | 0.7 | 0.779283 |
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Target: 5'- -aGUCCACAGUCAGG-GUGGAc---- -3' miRNA: 3'- agCAGGUGUCAGUCCgCGCUUuagag -5' |
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| 28545 | 3' | -52.8 | NC_005946.1 | + | 18340 | 0.74 | 0.548559 |
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Target: 5'- cCGUCCACagaggaucucaGGUCGGGgGUGAGAUC-Ca -3' miRNA: 3'- aGCAGGUG-----------UCAGUCCgCGCUUUAGaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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