miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28545 5' -54 NC_005946.1 + 88155 0.66 0.91739
Target:  5'- aCCGUgGCUCagGcCCUGuccaggGUGGUGGGg -3'
miRNA:   3'- -GGCA-UGAGagC-GGACuaa---CGCCACCU- -5'
28545 5' -54 NC_005946.1 + 86951 0.67 0.905093
Target:  5'- gCGUACUCgggaaCCUGGc-GUGGUGGAg -3'
miRNA:   3'- gGCAUGAGagc--GGACUaaCGCCACCU- -5'
28545 5' -54 NC_005946.1 + 19891 0.7 0.745102
Target:  5'- cCCGUGCUggagCUCauGCCUGAgUGC-GUGGAc -3'
miRNA:   3'- -GGCAUGA----GAG--CGGACUaACGcCACCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.