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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28545 | 5' | -54 | NC_005946.1 | + | 88155 | 0.66 | 0.91739 |
Target: 5'- aCCGUgGCUCagGcCCUGuccaggGUGGUGGGg -3' miRNA: 3'- -GGCA-UGAGagC-GGACuaa---CGCCACCU- -5' |
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28545 | 5' | -54 | NC_005946.1 | + | 86951 | 0.67 | 0.905093 |
Target: 5'- gCGUACUCgggaaCCUGGc-GUGGUGGAg -3' miRNA: 3'- gGCAUGAGagc--GGACUaaCGCCACCU- -5' |
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28545 | 5' | -54 | NC_005946.1 | + | 19891 | 0.7 | 0.745102 |
Target: 5'- cCCGUGCUggagCUCauGCCUGAgUGC-GUGGAc -3' miRNA: 3'- -GGCAUGA----GAG--CGGACUaACGcCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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