Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28546 | 5' | -56.3 | NC_005946.1 | + | 76553 | 0.66 | 0.81025 |
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Target: 5'- gGCugGGcCUCaGGGgCaGAGGGGAAu -3' miRNA: 3'- aCGugUCaGAG-CCCgGaCUUCCCUUc -5' |
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| 28546 | 5' | -56.3 | NC_005946.1 | + | 12760 | 0.66 | 0.81025 |
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Target: 5'- gGCGCuGUgCUCaGGGC--GGAGGGAGGc -3' miRNA: 3'- aCGUGuCA-GAG-CCCGgaCUUCCCUUC- -5' |
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| 28546 | 5' | -56.3 | NC_005946.1 | + | 99986 | 0.69 | 0.660902 |
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Target: 5'- cUGgACAGUCUUGGccuGCUUGAuGGGAGc -3' miRNA: 3'- -ACgUGUCAGAGCC---CGGACUuCCCUUc -5' |
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| 28546 | 5' | -56.3 | NC_005946.1 | + | 9086 | 0.7 | 0.566416 |
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Target: 5'- aGCAUuGUCUCGGaCCUGGccuGGGGAc- -3' miRNA: 3'- aCGUGuCAGAGCCcGGACU---UCCCUuc -5' |
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| 28546 | 5' | -56.3 | NC_005946.1 | + | 36262 | 0.74 | 0.351039 |
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Target: 5'- gGCgGCGGUggCGGGUCUGggGGGAGc -3' miRNA: 3'- aCG-UGUCAgaGCCCGGACuuCCCUUc -5' |
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| 28546 | 5' | -56.3 | NC_005946.1 | + | 10274 | 0.74 | 0.343066 |
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Target: 5'- gGCAUAGUCcgaCGGGCaccaGGAGGGAAGc -3' miRNA: 3'- aCGUGUCAGa--GCCCGga--CUUCCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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