Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28547 | 3' | -60.2 | NC_005946.1 | + | 102296 | 0.66 | 0.61663 |
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Target: 5'- -uGCAGGGccccGCACGCCAGGGc--- -3' miRNA: 3'- guCGUCCCcuc-UGUGCGGUCCCacua -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 24708 | 0.66 | 0.585703 |
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Target: 5'- cCGGUuuGGGGGGGuuGgGCCcGGGUGGg -3' miRNA: 3'- -GUCG--UCCCCUCugUgCGGuCCCACUa -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 49178 | 0.66 | 0.565244 |
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Target: 5'- gCAGcCAGGGGGGGCAUagGCCAagucaccaGGGUa-- -3' miRNA: 3'- -GUC-GUCCCCUCUGUG--CGGU--------CCCAcua -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 83896 | 0.67 | 0.524948 |
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Target: 5'- aGGCGGGGuGGAUGCaagaCAGGGUGAg -3' miRNA: 3'- gUCGUCCCcUCUGUGcg--GUCCCACUa -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 94034 | 0.67 | 0.505207 |
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Target: 5'- cCAGCAGGGGgaggAGGCuggugauUGCCAGGGc--- -3' miRNA: 3'- -GUCGUCCCC----UCUGu------GCGGUCCCacua -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 72838 | 0.68 | 0.485791 |
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Target: 5'- gGGCGGGguucucccuGGAGACAC-CCAGGGaGGUu -3' miRNA: 3'- gUCGUCC---------CCUCUGUGcGGUCCCaCUA- -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 79919 | 0.68 | 0.465795 |
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Target: 5'- aCGGCGGGGGGGACAauguuuaGCCAgcaccucaccagaGGGUc-- -3' miRNA: 3'- -GUCGUCCCCUCUGUg------CGGU-------------CCCAcua -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 4632 | 0.69 | 0.403319 |
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Target: 5'- aGGCGGuGGGGGuCACGCCAGaGUGc- -3' miRNA: 3'- gUCGUC-CCCUCuGUGCGGUCcCACua -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 12774 | 0.69 | 0.394712 |
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Target: 5'- aCAGguGGGGGGACGgCGCugugcuCAGGGcgGAg -3' miRNA: 3'- -GUCguCCCCUCUGU-GCG------GUCCCa-CUa -5' |
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| 28547 | 3' | -60.2 | NC_005946.1 | + | 21843 | 0.82 | 0.059077 |
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Target: 5'- gGGCAGGGGuGGCAcacugcCGCCAGGGUGGc -3' miRNA: 3'- gUCGUCCCCuCUGU------GCGGUCCCACUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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