Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28547 | 5' | -54.8 | NC_005946.1 | + | 100043 | 0.66 | 0.878923 |
Target: 5'- -gACCACaaaCAGCUCGUCcaUGUCCUg-- -3' miRNA: 3'- ugUGGUGg--GUCGAGUAG--AUAGGGugc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 104405 | 0.66 | 0.878923 |
Target: 5'- gGCGCagGCCCuGUU-GUCUAcUCCCACGg -3' miRNA: 3'- -UGUGg-UGGGuCGAgUAGAU-AGGGUGC- -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 10745 | 0.66 | 0.870668 |
Target: 5'- aGCACUugacgggAUCCAGCUCGaggcuugcagUCUuUCCCACc -3' miRNA: 3'- -UGUGG-------UGGGUCGAGU----------AGAuAGGGUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 73240 | 0.67 | 0.839214 |
Target: 5'- gACGCCACCgGGCcccUCAUCgccuUCCUccuggACGg -3' miRNA: 3'- -UGUGGUGGgUCG---AGUAGau--AGGG-----UGC- -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 2225 | 0.67 | 0.830635 |
Target: 5'- gACGCCGCCCugGGCUCcuUCUGUaucaagaaCCC-CGg -3' miRNA: 3'- -UGUGGUGGG--UCGAGu-AGAUA--------GGGuGC- -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 41697 | 0.67 | 0.812902 |
Target: 5'- uGCcCCugucCCCGGCUCcccCUgaGUCCCACGg -3' miRNA: 3'- -UGuGGu---GGGUCGAGua-GA--UAGGGUGC- -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 60366 | 0.68 | 0.775396 |
Target: 5'- aGCGCCGcCCCAGC-CAcUCccgcUCCCACa -3' miRNA: 3'- -UGUGGU-GGGUCGaGU-AGau--AGGGUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 39876 | 0.68 | 0.755776 |
Target: 5'- cGCACUGCa-GGCUCGUCguUCCCGCu -3' miRNA: 3'- -UGUGGUGggUCGAGUAGauAGGGUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 29397 | 0.69 | 0.745785 |
Target: 5'- gACACaAUCCAGCUCGUCUGugagacUCUCAUu -3' miRNA: 3'- -UGUGgUGGGUCGAGUAGAU------AGGGUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 11244 | 0.69 | 0.745785 |
Target: 5'- -gGCCGCCCuGCUCAgggGUCCgACc -3' miRNA: 3'- ugUGGUGGGuCGAGUagaUAGGgUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 104639 | 0.71 | 0.620442 |
Target: 5'- gACugUACCCAGCcUAUCUGU-CCACu -3' miRNA: 3'- -UGugGUGGGUCGaGUAGAUAgGGUGc -5' |
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28547 | 5' | -54.8 | NC_005946.1 | + | 40364 | 0.72 | 0.578167 |
Target: 5'- gACACCGCCgCGGCguaggaagaGUC-GUCCCACGc -3' miRNA: 3'- -UGUGGUGG-GUCGag-------UAGaUAGGGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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