Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28548 | 3' | -51.5 | NC_005946.1 | + | 4274 | 0.66 | 0.949469 |
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Target: 5'- gGACACgAGGCc--GUCGGGGGCUccCAc -3' miRNA: 3'- -UUGUG-UCUGuuuCAGUCCCCGAa-GUa -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 2289 | 0.68 | 0.889869 |
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Target: 5'- gGACGCGGACcccGcCGGGGGCUggugCAg -3' miRNA: 3'- -UUGUGUCUGuuuCaGUCCCCGAa---GUa -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 57805 | 0.69 | 0.841179 |
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Target: 5'- cAACGCAGAacuguuuGAGGaCAGGGGCUUUg- -3' miRNA: 3'- -UUGUGUCUg------UUUCaGUCCCCGAAGua -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 77857 | 0.7 | 0.804133 |
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Target: 5'- -uCACGGACugGAAGUCGGGGGU--CAUg -3' miRNA: 3'- uuGUGUCUG--UUUCAGUCCCCGaaGUA- -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 8595 | 0.7 | 0.794385 |
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Target: 5'- cAugACGGGCAcAGUCAGGuugucggcuauGGCUUCGg -3' miRNA: 3'- -UugUGUCUGUuUCAGUCC-----------CCGAAGUa -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 62126 | 0.7 | 0.774381 |
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Target: 5'- cGGCGCAGcCAGGGagAGGGGCUcCGUc -3' miRNA: 3'- -UUGUGUCuGUUUCagUCCCCGAaGUA- -5' |
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| 28548 | 3' | -51.5 | NC_005946.1 | + | 35180 | 0.73 | 0.645036 |
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Target: 5'- aGACACcagGGACGGAGaCGGGGGCaugUCAUa -3' miRNA: 3'- -UUGUG---UCUGUUUCaGUCCCCGa--AGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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