Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28549 | 3' | -56.5 | NC_005946.1 | + | 48614 | 0.66 | 0.823685 |
Target: 5'- -cCGCCCUcUCCUcGGCCUgcuCCUCGg -3' miRNA: 3'- uuGUGGGAcAGGAaCUGGGu--GGAGCg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 84463 | 0.66 | 0.805833 |
Target: 5'- uACAUCUccuucuUGUCC-UGACCCAUUaUCGCg -3' miRNA: 3'- uUGUGGG------ACAGGaACUGGGUGG-AGCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 47910 | 0.66 | 0.796654 |
Target: 5'- cGACucccuUCCUGUCCcUGGCCCucacCCUCa- -3' miRNA: 3'- -UUGu----GGGACAGGaACUGGGu---GGAGcg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 93994 | 0.66 | 0.787317 |
Target: 5'- gGACGCCCcggccGUCC-UGACUgACCUCc- -3' miRNA: 3'- -UUGUGGGa----CAGGaACUGGgUGGAGcg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 32506 | 0.66 | 0.777833 |
Target: 5'- uGACGuCCUUGUCCUUGAggugcuucuuuaCCCugcacucgaugGCCUCGa -3' miRNA: 3'- -UUGU-GGGACAGGAACU------------GGG-----------UGGAGCg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 40062 | 0.67 | 0.768212 |
Target: 5'- aAACACCCUGacaUCCgcgGAuCCCGCuCUCu- -3' miRNA: 3'- -UUGUGGGAC---AGGaa-CU-GGGUG-GAGcg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 20728 | 0.67 | 0.768212 |
Target: 5'- gGACGCCgUGgucaccagCC-UGGCCgGCCUCGa -3' miRNA: 3'- -UUGUGGgACa-------GGaACUGGgUGGAGCg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 49104 | 0.67 | 0.748595 |
Target: 5'- aAACACCCUGgCCUUc-CCCGCCcUGUc -3' miRNA: 3'- -UUGUGGGACaGGAAcuGGGUGGaGCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 36899 | 0.67 | 0.738619 |
Target: 5'- cAUACCacaCUGUCUgcGACCCGCagggCUCGCu -3' miRNA: 3'- uUGUGG---GACAGGaaCUGGGUG----GAGCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 80275 | 0.67 | 0.728546 |
Target: 5'- -cUACCCUGUaccucaCCUUGGCCaauGCCgCGCc -3' miRNA: 3'- uuGUGGGACA------GGAACUGGg--UGGaGCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 9629 | 0.67 | 0.718385 |
Target: 5'- aGACACCCcGUCCUcGucAUCCGCCgccucCGCc -3' miRNA: 3'- -UUGUGGGaCAGGAaC--UGGGUGGa----GCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 91895 | 0.68 | 0.708145 |
Target: 5'- aAACACCCagGUUCaccgGACCCugCUUGa -3' miRNA: 3'- -UUGUGGGa-CAGGaa--CUGGGugGAGCg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 11242 | 0.68 | 0.697838 |
Target: 5'- -cCGCCCUGcUCaggGGuCCgACCUCGCa -3' miRNA: 3'- uuGUGGGAC-AGgaaCU-GGgUGGAGCG- -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 3706 | 0.68 | 0.677058 |
Target: 5'- -uCGCCCUGUCC---ACCUGCCUCc- -3' miRNA: 3'- uuGUGGGACAGGaacUGGGUGGAGcg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 18155 | 0.72 | 0.471697 |
Target: 5'- --gGCCUUGgCCUUGACCUGCCUCc- -3' miRNA: 3'- uugUGGGACaGGAACUGGGUGGAGcg -5' |
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28549 | 3' | -56.5 | NC_005946.1 | + | 26423 | 0.73 | 0.404229 |
Target: 5'- gAGguCCCUGUCCUcgggagccggUuucaggcugcuccuGGCCCACCUCGCc -3' miRNA: 3'- -UUguGGGACAGGA----------A--------------CUGGGUGGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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