Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28551 | 3' | -56.5 | NC_005946.1 | + | 101322 | 0.66 | 0.836743 |
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Target: 5'- gCUUGACUGCUaCUCCA---AGUcCCUCAa -3' miRNA: 3'- -GGACUGACGA-GGGGUcugUCA-GGAGU- -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 8986 | 0.66 | 0.810691 |
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Target: 5'- gCCUGAUUGuCUUgCCGGGgAG-CCUCu -3' miRNA: 3'- -GGACUGAC-GAGgGGUCUgUCaGGAGu -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 21533 | 0.67 | 0.783121 |
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Target: 5'- gCUGgucACUGCgaCUCCaAGGCGGUUCUCAa -3' miRNA: 3'- gGAC---UGACGa-GGGG-UCUGUCAGGAGU- -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 17619 | 0.68 | 0.724429 |
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Target: 5'- gCCUGguaaaACUGCUgCUUGGACcuGGUCCUCu -3' miRNA: 3'- -GGAC-----UGACGAgGGGUCUG--UCAGGAGu -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 29050 | 0.68 | 0.7143 |
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Target: 5'- uCUUGugUGCUCCCCA---AGUCUUUu -3' miRNA: 3'- -GGACugACGAGGGGUcugUCAGGAGu -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 86155 | 0.69 | 0.641887 |
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Target: 5'- gCUGACcuugGuCUCUUCAG-CGGUCCUCAu -3' miRNA: 3'- gGACUGa---C-GAGGGGUCuGUCAGGAGU- -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 48080 | 0.71 | 0.54853 |
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Target: 5'- uCCUGuCUGCuuugUCCCCGGugGGUUCg-- -3' miRNA: 3'- -GGACuGACG----AGGGGUCugUCAGGagu -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 4199 | 0.71 | 0.54853 |
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Target: 5'- uCCUacGGCUGgaaccUUCCCCAGGgGGUCCUUg -3' miRNA: 3'- -GGA--CUGAC-----GAGGGGUCUgUCAGGAGu -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 12725 | 0.71 | 0.518229 |
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Target: 5'- gCUGuCUGCUCCCU--ACGGuUCCUCGg -3' miRNA: 3'- gGACuGACGAGGGGucUGUC-AGGAGU- -5' |
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| 28551 | 3' | -56.5 | NC_005946.1 | + | 52989 | 0.76 | 0.274164 |
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Target: 5'- --cGGCUGCUCgCCUAGACAGggagCCUCu -3' miRNA: 3'- ggaCUGACGAG-GGGUCUGUCa---GGAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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