Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28551 | 5' | -52.3 | NC_005946.1 | + | 12800 | 0.66 | 0.959374 |
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Target: 5'- gGUGACGG-CUGg--CAGGAGGUaCGu -3' miRNA: 3'- aCACUGUCuGAUaagGUCCUCCGaGC- -5' |
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| 28551 | 5' | -52.3 | NC_005946.1 | + | 557 | 0.66 | 0.955432 |
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Target: 5'- -cUGACGGAgggUUGUUCCAcuccacGGGGGCUUu -3' miRNA: 3'- acACUGUCU---GAUAAGGU------CCUCCGAGc -5' |
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| 28551 | 5' | -52.3 | NC_005946.1 | + | 82623 | 0.66 | 0.946788 |
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Target: 5'- -cUGACAGGCUGgacaugUCUgccGGAGGCUg- -3' miRNA: 3'- acACUGUCUGAUa-----AGGu--CCUCCGAgc -5' |
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| 28551 | 5' | -52.3 | NC_005946.1 | + | 26960 | 0.66 | 0.946788 |
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Target: 5'- gGUGGCGucAgUGUUCCAGGcGGGCUUc -3' miRNA: 3'- aCACUGUc-UgAUAAGGUCC-UCCGAGc -5' |
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| 28551 | 5' | -52.3 | NC_005946.1 | + | 71164 | 0.69 | 0.872916 |
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Target: 5'- cGUGGCGGGCaggUCCAGGgagggGGGCa-- -3' miRNA: 3'- aCACUGUCUGauaAGGUCC-----UCCGagc -5' |
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| 28551 | 5' | -52.3 | NC_005946.1 | + | 69018 | 0.7 | 0.794884 |
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Target: 5'- uUGUGGCGGGC---UCCAGGaAGGC-Ca -3' miRNA: 3'- -ACACUGUCUGauaAGGUCC-UCCGaGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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