Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28554 | 5' | -55.8 | NC_005946.1 | + | 43708 | 0.66 | 0.862358 |
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Target: 5'- aGCUCCGgcuugCCUCCGCuguacuuuUCggCCAGGGu -3' miRNA: 3'- gUGAGGCa----GGGGGUGuu------AGa-GGUCUC- -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 99229 | 0.66 | 0.829563 |
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Target: 5'- aGCUCCugucGUCCCuCCACAuUUUCCGcAGg -3' miRNA: 3'- gUGAGG----CAGGG-GGUGUuAGAGGUcUC- -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 66621 | 0.66 | 0.829563 |
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Target: 5'- gGCUCCGacgUCCUCGCGuug-CCAGAGa -3' miRNA: 3'- gUGAGGCa--GGGGGUGUuagaGGUCUC- -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 8565 | 0.68 | 0.715432 |
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Target: 5'- gGCUUCGggcaCCCCCACcucguccacCUCCAGGGu -3' miRNA: 3'- gUGAGGCa---GGGGGUGuua------GAGGUCUC- -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 92307 | 0.69 | 0.674159 |
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Target: 5'- cCACcUCGUaCCCCGCAGcCUCCAGAc -3' miRNA: 3'- -GUGaGGCAgGGGGUGUUaGAGGUCUc -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 94111 | 0.73 | 0.441273 |
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Target: 5'- gGCUCUGUCCUCCAgGAUC-CCAGc- -3' miRNA: 3'- gUGAGGCAGGGGGUgUUAGaGGUCuc -5' |
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| 28554 | 5' | -55.8 | NC_005946.1 | + | 89839 | 0.75 | 0.362609 |
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Target: 5'- ---aCCGUgCCCCCGuCAGUCUCCAGGu -3' miRNA: 3'- gugaGGCA-GGGGGU-GUUAGAGGUCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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