Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28556 | 3' | -49.8 | NC_005946.1 | + | 67775 | 0.66 | 0.991659 |
Target: 5'- gCCuGUCUUUgGGCAUGGCGUuuuccACCUc -3' miRNA: 3'- gGGuCAGGAAaUUGUGCCGUA-----UGGAc -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 25994 | 0.66 | 0.990923 |
Target: 5'- gCCCGGUCCUcuccucuacgcuaaaUUuACaaACGGCGUACa-- -3' miRNA: 3'- -GGGUCAGGA---------------AAuUG--UGCCGUAUGgac -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 60437 | 0.66 | 0.990405 |
Target: 5'- uCCCAGUCCc----CACGGCAggagACa-- -3' miRNA: 3'- -GGGUCAGGaaauuGUGCCGUa---UGgac -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 22557 | 0.66 | 0.989009 |
Target: 5'- aCCAGUCUgac--CACGGCG-ACCg- -3' miRNA: 3'- gGGUCAGGaaauuGUGCCGUaUGGac -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 20964 | 0.66 | 0.989009 |
Target: 5'- uCCCAGUCauacGugGUGGCGgACCUGg -3' miRNA: 3'- -GGGUCAGgaaaUugUGCCGUaUGGAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 28996 | 0.66 | 0.987129 |
Target: 5'- aUCAGUCCUacucUUGcugcucggcuccCACGGCGUACcCUGg -3' miRNA: 3'- gGGUCAGGA----AAUu-----------GUGCCGUAUG-GAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 30057 | 0.67 | 0.977057 |
Target: 5'- -gCGGUCCUa-AACAUGGCAgaUGCCa- -3' miRNA: 3'- ggGUCAGGAaaUUGUGCCGU--AUGGac -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 8888 | 0.67 | 0.977057 |
Target: 5'- aCCAGacaCCUUgu-CACGGuCAUGCCg- -3' miRNA: 3'- gGGUCa--GGAAauuGUGCC-GUAUGGac -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 62745 | 0.68 | 0.969631 |
Target: 5'- uCCCAGUcucggaaccgguggcCCUcUUGACgACGGCGUcguccuucuucaGCCUGa -3' miRNA: 3'- -GGGUCA---------------GGA-AAUUG-UGCCGUA------------UGGAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 76366 | 0.68 | 0.964989 |
Target: 5'- aCCGGUCCc--AGCGgGGC--GCCUGg -3' miRNA: 3'- gGGUCAGGaaaUUGUgCCGuaUGGAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 78974 | 0.68 | 0.961382 |
Target: 5'- gCCGGgggCCacagGGCuuGGCGUACCUGc -3' miRNA: 3'- gGGUCa--GGaaa-UUGugCCGUAUGGAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 64267 | 0.71 | 0.875653 |
Target: 5'- gUCAGUCC----ACACGGCGUcagaggccGCCUGa -3' miRNA: 3'- gGGUCAGGaaauUGUGCCGUA--------UGGAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 47820 | 0.72 | 0.867835 |
Target: 5'- gUCCAGUCCcgcagacCugGGCAUACaCUGu -3' miRNA: 3'- -GGGUCAGGaaauu--GugCCGUAUG-GAC- -5' |
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28556 | 3' | -49.8 | NC_005946.1 | + | 84030 | 0.72 | 0.842098 |
Target: 5'- cUCCAGUCCUgaccgacGugGCGGCgggaaggagggugGUGCCUGc -3' miRNA: 3'- -GGGUCAGGAaa-----UugUGCCG-------------UAUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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