Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28556 | 5' | -56.8 | NC_005946.1 | + | 14134 | 0.66 | 0.753536 |
Target: 5'- uGGaCGAgGUGCACCAccucagGUCAGugcAGGCCGAa -3' miRNA: 3'- -UC-GCU-CAUGUGGUa-----CGGUC---UCCGGUU- -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 104039 | 0.67 | 0.712676 |
Target: 5'- uGGCuccagACugUAUGUCGGAGGCCAc -3' miRNA: 3'- -UCGcuca-UGugGUACGGUCUCCGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 7329 | 0.67 | 0.712676 |
Target: 5'- uGCGAGUGCACCGcgacUGCCGuGGuguauGCCGu -3' miRNA: 3'- uCGCUCAUGUGGU----ACGGUcUC-----CGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 98452 | 0.67 | 0.702249 |
Target: 5'- cAGCGAGUaguacucgACucCCAUGUCGGGGaGCCu- -3' miRNA: 3'- -UCGCUCA--------UGu-GGUACGGUCUC-CGGuu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 2915 | 0.67 | 0.702249 |
Target: 5'- cAGCGAGgauCGCCucgGauaUAGAGGCCGc -3' miRNA: 3'- -UCGCUCau-GUGGua-Cg--GUCUCCGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 14448 | 0.68 | 0.628021 |
Target: 5'- aGGCaaGAcGUGCACCGUgGUCAGuguGGCCGAg -3' miRNA: 3'- -UCG--CU-CAUGUGGUA-CGGUCu--CCGGUU- -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 23612 | 0.68 | 0.626954 |
Target: 5'- cGGCGgugaggGGUACGaaaggagUCAUGCCAGuGGCCGc -3' miRNA: 3'- -UCGC------UCAUGU-------GGUACGGUCuCCGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 48122 | 0.69 | 0.596071 |
Target: 5'- aGGUGGGUAgAaagAUGUCGGGGGCCAGg -3' miRNA: 3'- -UCGCUCAUgUgg-UACGGUCUCCGGUU- -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 14961 | 0.69 | 0.596071 |
Target: 5'- gGGCGGGguagacggGCACUGUGCCGGcGGCa-- -3' miRNA: 3'- -UCGCUCa-------UGUGGUACGGUCuCCGguu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 105565 | 0.7 | 0.533148 |
Target: 5'- -cCGAGUACAUUGUGCCGuccAGGCCAc -3' miRNA: 3'- ucGCUCAUGUGGUACGGUc--UCCGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 26652 | 0.7 | 0.492527 |
Target: 5'- uGGCcccGUACACCA-GCCAGGGGaCCGu -3' miRNA: 3'- -UCGcu-CAUGUGGUaCGGUCUCC-GGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 86996 | 0.7 | 0.482592 |
Target: 5'- aGGaCGGGUucgGCGCCcgGCCuGGGGUCAAa -3' miRNA: 3'- -UC-GCUCA---UGUGGuaCGGuCUCCGGUU- -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 14196 | 0.73 | 0.347208 |
Target: 5'- gAGCGuGgcCACCAUGagcgCGGAGGCCAu -3' miRNA: 3'- -UCGCuCauGUGGUACg---GUCUCCGGUu -5' |
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28556 | 5' | -56.8 | NC_005946.1 | + | 78991 | 0.85 | 0.060965 |
Target: 5'- aGGCGGcGUACGCCAggGCCGGGGGCCAc -3' miRNA: 3'- -UCGCU-CAUGUGGUa-CGGUCUCCGGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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