Results 21 - 31 of 31 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28557 | 5' | -51.5 | NC_005946.1 | + | 94041 | 0.67 | 0.939236 |
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Target: 5'- aUCAGGAccAGCAGggggaggAGGCUGgugauugccAGGGCCc -3' miRNA: 3'- -AGUCCUa-UUGUCag-----UUCGAC---------UCCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 14450 | 0.67 | 0.943654 |
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Target: 5'- aCAGGcaagacgugcaccGUGGuCAGUguGGCcgaGAGGGCCa -3' miRNA: 3'- aGUCC-------------UAUU-GUCAguUCGa--CUCCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 76428 | 0.67 | 0.948763 |
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Target: 5'- aCAGGGguccaGACGGUUuGGCcggaaUGAgGGGCCu -3' miRNA: 3'- aGUCCUa----UUGUCAGuUCG-----ACU-CCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 76643 | 0.67 | 0.953132 |
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Target: 5'- cCAGGGgccCAGggGAGCaGAGGGaCCa -3' miRNA: 3'- aGUCCUauuGUCagUUCGaCUCCC-GG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 10335 | 0.66 | 0.956843 |
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Target: 5'- uUCAGGAUGACGGaggCGuGCUugaggaaGAGGGaCUc -3' miRNA: 3'- -AGUCCUAUUGUCa--GUuCGA-------CUCCC-GG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 67727 | 0.66 | 0.957243 |
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Target: 5'- cCAGGGccaccACGGugaccUCGGGCUGAGGGaCg -3' miRNA: 3'- aGUCCUau---UGUC-----AGUUCGACUCCCgG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 28299 | 0.66 | 0.957243 |
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Target: 5'- cUCGGGGuUGGCGGaaccGGCaGAGGGCUu -3' miRNA: 3'- -AGUCCU-AUUGUCagu-UCGaCUCCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 21395 | 0.66 | 0.961101 |
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Target: 5'- -aGGGAUGacGCAGUCAGGgUaaaGAGGGa- -3' miRNA: 3'- agUCCUAU--UGUCAGUUCgA---CUCCCgg -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 13319 | 0.66 | 0.96471 |
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Target: 5'- cCAGGGU---GGU--GGCcGAGGGCCu -3' miRNA: 3'- aGUCCUAuugUCAguUCGaCUCCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 65338 | 0.66 | 0.970909 |
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Target: 5'- cCAGcacucUGACcaagcagAGUCGGGCgucGAGGGCCa -3' miRNA: 3'- aGUCcu---AUUG-------UCAGUUCGa--CUCCCGG- -5' |
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| 28557 | 5' | -51.5 | NC_005946.1 | + | 12829 | 0.66 | 0.971212 |
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Target: 5'- -aAGGugGACAuGUCcacGCUGuGGGCCg -3' miRNA: 3'- agUCCuaUUGU-CAGuu-CGACuCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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