Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28558 | 3' | -58.5 | NC_005946.1 | + | 97867 | 0.76 | 0.233976 |
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Target: 5'- gCUCCGUCcUGGCCUgUGGCCgggGCGGg -3' miRNA: 3'- -GGGGUAGcACCGGAgGCUGGaa-CGCC- -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 36010 | 0.72 | 0.403283 |
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Target: 5'- gCCUCAUacggaGUGGCCUCCagGGCCUcgGCGu -3' miRNA: 3'- -GGGGUAg----CACCGGAGG--CUGGAa-CGCc -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 28745 | 0.72 | 0.403283 |
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Target: 5'- -gCCAUgGUGGCCUCCaucACCUUG-GGa -3' miRNA: 3'- ggGGUAgCACCGGAGGc--UGGAACgCC- -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 52921 | 0.66 | 0.741835 |
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Target: 5'- cUCCCGUCGUGagaGCCUCCGcucCCgucGUGa -3' miRNA: 3'- -GGGGUAGCAC---CGGAGGCu--GGaa-CGCc -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 4326 | 0.66 | 0.741835 |
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Target: 5'- aCCCCG-CGUGGUaguuuagUUCGACC--GCGGa -3' miRNA: 3'- -GGGGUaGCACCGg------AGGCUGGaaCGCC- -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 4967 | 0.66 | 0.770265 |
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Target: 5'- -gCCAUCa-GGCCUCCGACaacuacgGCGa -3' miRNA: 3'- ggGGUAGcaCCGGAGGCUGgaa----CGCc -5' |
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| 28558 | 3' | -58.5 | NC_005946.1 | + | 79204 | 0.66 | 0.779512 |
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Target: 5'- uCUCCAUCGgaaGGCCuuUCgGGCCUcuUGCu- -3' miRNA: 3'- -GGGGUAGCa--CCGG--AGgCUGGA--ACGcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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