Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28559 | 5' | -54.4 | NC_005946.1 | + | 36016 | 0.66 | 0.912355 |
Target: 5'- uCCCUGGccucaUACggaGUgGCCUCCAgggCCUc -3' miRNA: 3'- -GGGACC-----AUGauaCGgUGGAGGUa--GGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 62155 | 0.66 | 0.912355 |
Target: 5'- gUCCUGGg---AUGCaaacCGCCUCCcgCUCa -3' miRNA: 3'- -GGGACCaugaUACG----GUGGAGGuaGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 86239 | 0.66 | 0.911129 |
Target: 5'- cCCCagGGaGCUcaccaucUGCaugacacccagaGCCUCCAUCCCg -3' miRNA: 3'- -GGGa-CCaUGAu------ACGg-----------UGGAGGUAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 98160 | 0.66 | 0.906126 |
Target: 5'- uCCCUG--ACUGUGCUGCucauggccuguCUCuCGUCCCc -3' miRNA: 3'- -GGGACcaUGAUACGGUG-----------GAG-GUAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 102010 | 0.66 | 0.899649 |
Target: 5'- gCUCgGGgGCc-UGCuCGCCUCCGUCCUu -3' miRNA: 3'- -GGGaCCaUGauACG-GUGGAGGUAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 41502 | 0.67 | 0.885962 |
Target: 5'- cCCUUGGacCUGUGCCGCCaCgGggaCCCg -3' miRNA: 3'- -GGGACCauGAUACGGUGGaGgUa--GGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 44862 | 0.67 | 0.871328 |
Target: 5'- aCCCUGGUcCUGUggacauagGCCG-CUCCAaUCUCu -3' miRNA: 3'- -GGGACCAuGAUA--------CGGUgGAGGU-AGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 56338 | 0.67 | 0.871328 |
Target: 5'- gCCUGGaggACgcUGCCcugguccucGCgUCCAUCCUg -3' miRNA: 3'- gGGACCa--UGauACGG---------UGgAGGUAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 28754 | 0.67 | 0.871328 |
Target: 5'- uUCUGGUAg---GCCauggugGCCUCCAUCaCCu -3' miRNA: 3'- gGGACCAUgauaCGG------UGGAGGUAG-GG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 5826 | 0.67 | 0.862889 |
Target: 5'- aCUCUGGgaaggggGgUAUGCCACaacagcuCUCCAgagUCCCc -3' miRNA: 3'- -GGGACCa------UgAUACGGUG-------GAGGU---AGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 42188 | 0.68 | 0.847691 |
Target: 5'- aCCCUGGggccACUcggGCCACUcgggCCcacGUCCCc -3' miRNA: 3'- -GGGACCa---UGAua-CGGUGGa---GG---UAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 23721 | 0.68 | 0.839389 |
Target: 5'- aCCCUGuccagGCUAaagucUGCgGCCUCCAUggCCUg -3' miRNA: 3'- -GGGACca---UGAU-----ACGgUGGAGGUA--GGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 45084 | 0.68 | 0.822196 |
Target: 5'- gCCCUGGcACagGUGCCucuGCCUCUcUgCCg -3' miRNA: 3'- -GGGACCaUGa-UACGG---UGGAGGuAgGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 4676 | 0.68 | 0.804273 |
Target: 5'- gCCCaugugguaaaUGGUGUUGUccaGCCACCUCCuGUCCa -3' miRNA: 3'- -GGG----------ACCAUGAUA---CGGUGGAGG-UAGGg -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 69385 | 0.69 | 0.78569 |
Target: 5'- gCCUUGGccACg--GCCACCgCCuuGUCCCa -3' miRNA: 3'- -GGGACCa-UGauaCGGUGGaGG--UAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 81659 | 0.69 | 0.766523 |
Target: 5'- gCCCUGGacgACUAUGCCGCaaagAUCaCCa -3' miRNA: 3'- -GGGACCa--UGAUACGGUGgaggUAG-GG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 13568 | 0.7 | 0.736851 |
Target: 5'- aCCUGGUGagg-GUgGCCUCguCAUCCCc -3' miRNA: 3'- gGGACCAUgauaCGgUGGAG--GUAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 52943 | 0.71 | 0.685603 |
Target: 5'- uCCCUGGgcgcgaUGCUGUccGCUcccgucgugagaGCCUCCGcUCCCg -3' miRNA: 3'- -GGGACC------AUGAUA--CGG------------UGGAGGU-AGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 68420 | 0.71 | 0.654214 |
Target: 5'- aCCCUGcacACUuuUGCCAUCUCUgugaGUCCCa -3' miRNA: 3'- -GGGACca-UGAu-ACGGUGGAGG----UAGGG- -5' |
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28559 | 5' | -54.4 | NC_005946.1 | + | 94383 | 0.72 | 0.61214 |
Target: 5'- gCCCUGGcguacACUGUgGCCAgagCCGUCCCa -3' miRNA: 3'- -GGGACCa----UGAUA-CGGUggaGGUAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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