Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28560 | 3' | -55.6 | NC_005946.1 | + | 103004 | 0.66 | 0.862385 |
Target: 5'- gGAGCAaaGGgAGGGCCg---GGAGa -3' miRNA: 3'- aCUCGUagCUgUCCCGGagaaCCUCg -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 79464 | 0.66 | 0.862385 |
Target: 5'- aGuGCGUCGAgGgguGGGCCgggUGGAcGCu -3' miRNA: 3'- aCuCGUAGCUgU---CCCGGagaACCU-CG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 20795 | 0.66 | 0.854428 |
Target: 5'- aGaAGC-UCGACAGG--CUCcUGGAGCa -3' miRNA: 3'- aC-UCGuAGCUGUCCcgGAGaACCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 97002 | 0.66 | 0.846256 |
Target: 5'- aGGGCGUUgcugcugcugGGCAGGGCgUCUcuGAGUc -3' miRNA: 3'- aCUCGUAG----------CUGUCCCGgAGAacCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 76291 | 0.66 | 0.837877 |
Target: 5'- gGAGacgacgguccCGUCGGCccaaAGGGaCCUCagGGAGCc -3' miRNA: 3'- aCUC----------GUAGCUG----UCCC-GGAGaaCCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 36349 | 0.66 | 0.837877 |
Target: 5'- gUGGGCcgCGGCGGcGGCCcacUCUgGGAa- -3' miRNA: 3'- -ACUCGuaGCUGUC-CCGG---AGAaCCUcg -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 36271 | 0.67 | 0.811573 |
Target: 5'- gUGGGCuccggCGGCGGuGGCgggUCUggggGGAGCg -3' miRNA: 3'- -ACUCGua---GCUGUC-CCGg--AGAa---CCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 85019 | 0.67 | 0.811573 |
Target: 5'- aUGGGCAU-GAUggAGGGCaUCagGGAGCu -3' miRNA: 3'- -ACUCGUAgCUG--UCCCGgAGaaCCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 58148 | 0.67 | 0.798748 |
Target: 5'- cUGAGCAagGACAGGGCagagaagCUCgucaagcacuacacaGGAGUg -3' miRNA: 3'- -ACUCGUagCUGUCCCG-------GAGaa-------------CCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 3898 | 0.67 | 0.783706 |
Target: 5'- ------cCGACAGGGCCUCgccgaauaugGGGGUa -3' miRNA: 3'- acucguaGCUGUCCCGGAGaa--------CCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 46269 | 0.67 | 0.774114 |
Target: 5'- cUGAGCGUUGACAuuguccuucuuGGCCUCUgguUGGAc- -3' miRNA: 3'- -ACUCGUAGCUGUc----------CCGGAGA---ACCUcg -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 78988 | 0.67 | 0.764385 |
Target: 5'- -cGGCGUaCGcCAGGGCCg---GGGGCc -3' miRNA: 3'- acUCGUA-GCuGUCCCGGagaaCCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 73575 | 0.68 | 0.754532 |
Target: 5'- aGAGCAU-GGCGGcccuCCUCaUGGAGCa -3' miRNA: 3'- aCUCGUAgCUGUCcc--GGAGaACCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 2477 | 0.7 | 0.609042 |
Target: 5'- gUGAGCGcCGAguGGGaCUCgaggGGGGCc -3' miRNA: 3'- -ACUCGUaGCUguCCCgGAGaa--CCUCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 28594 | 0.71 | 0.546313 |
Target: 5'- -uGGCGUCGGcCGGGGCCg--UGGuGCc -3' miRNA: 3'- acUCGUAGCU-GUCCCGGagaACCuCG- -5' |
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28560 | 3' | -55.6 | NC_005946.1 | + | 76561 | 0.72 | 0.515692 |
Target: 5'- gUGGGag-CGGCuGGGCCUCa-GGGGCa -3' miRNA: 3'- -ACUCguaGCUGuCCCGGAGaaCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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