Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28562 | 3' | -53.4 | NC_005946.1 | + | 93880 | 0.66 | 0.935008 |
Target: 5'- cCCGUGGCCGCagCGGuagaagagccCAGGGGAcAGGCc -3' miRNA: 3'- -GGUACUGGUG--GCCu---------GUCUCUU-UCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 14194 | 0.66 | 0.935008 |
Target: 5'- gCGUGGCCACCaugagcGCGGAGGccaucAGGGCc -3' miRNA: 3'- gGUACUGGUGGcc----UGUCUCU-----UUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 72120 | 0.66 | 0.929776 |
Target: 5'- -gGUGGCCAUCauGACGGcGGGAGGCu -3' miRNA: 3'- ggUACUGGUGGc-CUGUCuCUUUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 73960 | 0.67 | 0.92429 |
Target: 5'- gCCGUGugCuggACCaGGACAGAG--GGGUc -3' miRNA: 3'- -GGUACugG---UGG-CCUGUCUCuuUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 76676 | 0.67 | 0.92429 |
Target: 5'- gCAUGACCcCCGGGgaugacggcaacCAGGGGcccaGGGGCc -3' miRNA: 3'- gGUACUGGuGGCCU------------GUCUCU----UUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 21259 | 0.67 | 0.923727 |
Target: 5'- gCAUGGCCACCGucuuugaGACGGcAGAcucucacGGCGu -3' miRNA: 3'- gGUACUGGUGGC-------CUGUC-UCUuu-----CCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 38511 | 0.67 | 0.918549 |
Target: 5'- aCUAUGucuuCCAa-GGACAGAGucguGAGGCu -3' miRNA: 3'- -GGUACu---GGUggCCUGUCUCu---UUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 72800 | 0.67 | 0.918549 |
Target: 5'- --cUGGCCACCGucaGGCuGGGAAAGGg- -3' miRNA: 3'- gguACUGGUGGC---CUGuCUCUUUCCgc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 88008 | 0.67 | 0.906305 |
Target: 5'- aCCGUGACCAU--GAC-GAGAAAGGg- -3' miRNA: 3'- -GGUACUGGUGgcCUGuCUCUUUCCgc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 82557 | 0.67 | 0.893061 |
Target: 5'- aCCAcgacUGcACCACCGccucgucCGGGGAGAGGCu -3' miRNA: 3'- -GGU----AC-UGGUGGCcu-----GUCUCUUUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 14019 | 0.67 | 0.893061 |
Target: 5'- cCCAUGACCAaCGaGCcuGGAGAGcuGGCGg -3' miRNA: 3'- -GGUACUGGUgGCcUG--UCUCUUu-CCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 14655 | 0.68 | 0.886071 |
Target: 5'- cCCcUGGCUcaGCCGGGgGGAGGccgacaAGGGCa -3' miRNA: 3'- -GGuACUGG--UGGCCUgUCUCU------UUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 98616 | 0.68 | 0.886071 |
Target: 5'- gCCGUGGuggccUCAgCGGACAGGGugacguuAAGGCu -3' miRNA: 3'- -GGUACU-----GGUgGCCUGUCUCu------UUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 12756 | 0.68 | 0.886071 |
Target: 5'- --cUGugCucaGGGCGGAGGGAGGCa -3' miRNA: 3'- gguACugGuggCCUGUCUCUUUCCGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 3293 | 0.68 | 0.878842 |
Target: 5'- uCCAggcucaGGCCAUCGGcAguGAGGcuGGCGg -3' miRNA: 3'- -GGUa-----CUGGUGGCC-UguCUCUuuCCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 12877 | 0.68 | 0.863686 |
Target: 5'- gCCGUGGCUcaggugagGCUGGACGGc-GGAGGUGa -3' miRNA: 3'- -GGUACUGG--------UGGCCUGUCucUUUCCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 83735 | 0.69 | 0.813113 |
Target: 5'- aCGUGGCCGUgGGcGCGGGuGggGGGCGa -3' miRNA: 3'- gGUACUGGUGgCC-UGUCU-CuuUCCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 16431 | 0.7 | 0.794763 |
Target: 5'- aUCAaGGCCGCCGGAgCAGAGGAGauGuGCu -3' miRNA: 3'- -GGUaCUGGUGGCCU-GUCUCUUU--C-CGc -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 40662 | 0.7 | 0.785348 |
Target: 5'- aUCAUGACUGCCacaacugcaGACAGGGccacGAGGGCGg -3' miRNA: 3'- -GGUACUGGUGGc--------CUGUCUC----UUUCCGC- -5' |
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28562 | 3' | -53.4 | NC_005946.1 | + | 93939 | 0.71 | 0.736272 |
Target: 5'- cCCGcGACCGCCaGccuCAGAGAGuGGCGu -3' miRNA: 3'- -GGUaCUGGUGGcCu--GUCUCUUuCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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